Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK(toxin)
Location 3576323..3576912 Replicon chromosome
Accession NZ_CP082922
Organism Nocardioides rotundus strain MCCC 1A10561

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag K8W59_RS17645 Protein ID WP_223396262.1
Coordinates 3576323..3576673 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag K8W59_RS17650 Protein ID WP_223396263.1
Coordinates 3576667..3576912 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8W59_RS17615 (K8W59_17665) 3572114..3573313 + 1200 WP_223396257.1 thiolase family protein -
K8W59_RS17620 (K8W59_17670) 3573310..3574002 + 693 WP_223396258.1 3-oxoacid CoA-transferase subunit A -
K8W59_RS17625 (K8W59_17675) 3573999..3574634 + 636 WP_223396259.1 3-oxoacid CoA-transferase subunit B -
K8W59_RS17630 (K8W59_17680) 3574674..3575291 - 618 WP_223396260.1 HAD-IA family hydrolase -
K8W59_RS17635 (K8W59_17685) 3575288..3575731 - 444 WP_223399894.1 nucleoside deaminase -
K8W59_RS17640 (K8W59_17690) 3575758..3576258 - 501 WP_223396261.1 tRNA adenosine deaminase-associated protein -
K8W59_RS17645 (K8W59_17695) 3576323..3576673 - 351 WP_223396262.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
K8W59_RS17650 (K8W59_17700) 3576667..3576912 - 246 WP_223396263.1 hypothetical protein Antitoxin
K8W59_RS17655 (K8W59_17705) 3576991..3577632 + 642 WP_223396264.1 uracil phosphoribosyltransferase -
K8W59_RS17660 (K8W59_17710) 3577770..3579299 + 1530 WP_223396265.1 Re/Si-specific NAD(P)(+) transhydrogenase subunit alpha -
K8W59_RS17665 (K8W59_17715) 3579304..3580740 + 1437 WP_223396266.1 Re/Si-specific NAD(P)(+) transhydrogenase subunit beta -
K8W59_RS17670 (K8W59_17720) 3580856..3581419 - 564 WP_223396267.1 RNA 2',3'-cyclic phosphodiesterase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12294.03 Da        Isoelectric Point: 9.1612

>T215588 WP_223396262.1 NZ_CP082922:c3576673-3576323 [Nocardioides rotundus]
VVRRGDIHWVDLGRPDGGSAPAKRRPVVIIQSDDFNRSRLATTIAVVVTSNTALAAMPGNVFLPTAATGLSRDSVANVTA
LVTLDRRDLDPHPAGQVPGHLLDEIAEGIRRVLALS

Download         Length: 351 bp

>T215588 NZ_CP110816:3596195-3596473 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8710.47 Da        Isoelectric Point: 4.3637

>AT215588 WP_223396263.1 NZ_CP082922:c3576912-3576667 [Nocardioides rotundus]
VKTAISVPDRTFERAERAAQRHGLNRSQLYATAVERYLDELEAGDLTAAIDAVVEVANADHATGDAASAGRRVVADGDEE
W

Download         Length: 246 bp

>AT215588 NZ_CP110816:3595932-3596192 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References