Detailed information of TA system TA215587

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 3455768..3456405
• Replicon: chromosome
• Accession: NZ_CP082922
• Organism: Nocardioides rotundus strain MCCC 1A10561

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K8W59_RS17030
• Protein ID: WP_223396146.1
• Coordinates: 3456013..3456405 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K8W59_RS17025
• Protein ID: WP_223399890.1
• Coordinates: 3455768..3456016 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8W59_RS17015 (K8W59_17065)	3452013..3454739	-	2727	WP_223396144.1	type I DNA topoisomerase	-
K8W59_RS17020 (K8W59_17070)	3454896..3455726	+	831	WP_223396145.1	hypothetical protein	-
K8W59_RS17025 (K8W59_17075)	3455768..3456016	+	249	WP_223399890.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K8W59_RS17030 (K8W59_17080)	3456013..3456405	+	393	WP_223396146.1	type II toxin-antitoxin system VapC family toxin	Toxin
K8W59_RS17035 (K8W59_17085)	3456374..3457876	-	1503	WP_223396147.1	methyltransferase	-
K8W59_RS17040 (K8W59_17090)	3457915..3460215	-	2301	WP_223396148.1	sodium-translocating pyrophosphatase	-
K8W59_RS17045 (K8W59_17095)	3460357..3460686	-	330	WP_223396149.1	STAS domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Antitoxin VapB-like (2-81)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14053.04 Da
• Isoelectric Point: 5.6254

>T215587 WP_223396146.1 NZ_CP082922:3456013-3456405 [Nocardioides rotundus]
VIVDSSALVAIAYAEPEAAQLLGVLRTADRRRIAAPTVLESSMVLWRVAHRVADLVGQLDLEIVAFDADHLRVAQDAFIR
YGRGTGSPARLNFGDCISYALASATGEPLLFKGEDLRHTDITPAWTPPGR

Nucleotide

• Length: 393 bp

>T215587 NZ_CP110816:c3590133-3589735 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9278.41 Da
• Isoelectric Point: 5.1751

>AT215587 WP_223399890.1 NZ_CP082922:3455768-3456016 [Nocardioides rotundus]
MNIKDQRIHAAAQELARRRGVSMTEAVRQAVDEALARSAQTRDEAWVERILTLGRELRAEMDAAGARPMTDDDLYDERGV
PR

Nucleotide

• Length: 249 bp

>AT215587 NZ_CP110816:c3590429-3590136 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA