Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1693745..1694355 Replicon chromosome
Accession NZ_CP082922
Organism Nocardioides rotundus strain MCCC 1A10561

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag K8W59_RS08460 Protein ID WP_223399408.1
Coordinates 1693745..1694149 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag K8W59_RS08465 Protein ID WP_223399409.1
Coordinates 1694146..1694355 (-) Length 70 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8W59_RS08435 (K8W59_08400) 1688820..1689371 + 552 WP_223399403.1 amino acid synthesis family protein -
K8W59_RS08440 (K8W59_08405) 1689368..1690165 + 798 WP_223399404.1 alpha/beta hydrolase -
K8W59_RS08445 (K8W59_08410) 1690149..1691630 + 1482 WP_223399405.1 aldehyde dehydrogenase -
K8W59_RS08450 (K8W59_08415) 1691697..1692740 + 1044 WP_223399406.1 LLM class flavin-dependent oxidoreductase -
K8W59_RS08455 (K8W59_08420) 1692827..1693318 - 492 WP_223399407.1 hypothetical protein -
K8W59_RS08460 (K8W59_08425) 1693745..1694149 - 405 WP_223399408.1 PIN domain nuclease Toxin
K8W59_RS08465 (K8W59_08430) 1694146..1694355 - 210 WP_223399409.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K8W59_RS08470 (K8W59_08435) 1694470..1694694 - 225 WP_223399410.1 hypothetical protein -
K8W59_RS08475 (K8W59_08440) 1694800..1695009 - 210 WP_223399411.1 type II toxin-antitoxin system VapB family antitoxin -
K8W59_RS08480 (K8W59_08445) 1695088..1695603 + 516 WP_223399412.1 GNAT family N-acetyltransferase -
K8W59_RS08485 (K8W59_08450) 1695593..1696240 - 648 WP_223399413.1 maleylpyruvate isomerase family mycothiol-dependent enzyme -
K8W59_RS08490 (K8W59_08455) 1696310..1697002 + 693 WP_223399414.1 M50 family metallopeptidase -
K8W59_RS08495 1697261..1697365 - 105 WP_223399415.1 type II toxin-antitoxin system VapB family antitoxin -
K8W59_RS08500 (K8W59_08465) 1697413..1697838 - 426 WP_223399416.1 PPOX class F420-dependent oxidoreductase -
K8W59_RS08505 (K8W59_08470) 1697927..1698292 + 366 WP_223399417.1 DUF488 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1683491..1694504 11013


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-121)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(3-38)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14770.86 Da        Isoelectric Point: 4.5288

>T215585 WP_223399408.1 NZ_CP082922:c1694149-1693745 [Nocardioides rotundus]
VIVDTSVWIDFLRPGSSAAGDRLEEMVTTGDRIVVPETVLMELLSGPTDEIVAAQRQRMLEAFYLEPVVPLADSVRAASL
QRSCRRGGDTVRNLGDCLIAATALRLDLPVVHRDRDFEVLARHSDLRTESLLDA

Download         Length: 405 bp

>T215585 NZ_CP110816:3400110-3400328 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 70 a.a.        Molecular weight: 7886.97 Da        Isoelectric Point: 4.6920

>AT215585 WP_223399409.1 NZ_CP082922:c1694355-1694146 [Nocardioides rotundus]
MTRTNIDLDDELVSAVMRRYRLTSKKSAVDFALRQLVVTPMTRDEVLAMRGSGIEVSNDQIEGDWTIDR

Download         Length: 210 bp

>AT215585 NZ_CP110816:3399710-3400084 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References