Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 827377..827942 Replicon chromosome
Accession NZ_CP082922
Organism Nocardioides rotundus strain MCCC 1A10561

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag K8W59_RS03975 Protein ID WP_223399724.1
Coordinates 827377..827727 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag K8W59_RS03980 Protein ID WP_223397461.1
Coordinates 827724..827942 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8W59_RS03945 (K8W59_03940) 823180..824016 - 837 WP_223397456.1 molybdate ABC transporter permease subunit -
K8W59_RS03950 (K8W59_03945) 824013..824768 - 756 WP_223397457.1 molybdate ABC transporter substrate-binding protein -
K8W59_RS03955 (K8W59_03950) 824782..825186 - 405 WP_223397458.1 helix-turn-helix domain-containing protein -
K8W59_RS03960 (K8W59_03955) 825344..825907 + 564 WP_223397459.1 DUF4245 family protein -
K8W59_RS03965 (K8W59_03960) 825882..826100 - 219 WP_223397460.1 exodeoxyribonuclease VII small subunit -
K8W59_RS03970 (K8W59_03965) 826093..827328 - 1236 WP_263283280.1 exodeoxyribonuclease VII large subunit -
K8W59_RS03975 (K8W59_03970) 827377..827727 - 351 WP_223399724.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
K8W59_RS03980 (K8W59_03975) 827724..827942 - 219 WP_223397461.1 ribbon-helix-helix domain-containing protein Antitoxin
K8W59_RS03985 (K8W59_03980) 828066..829067 + 1002 WP_223397462.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
K8W59_RS03990 (K8W59_03985) 829075..829698 + 624 WP_223397463.1 L-threonylcarbamoyladenylate synthase -
K8W59_RS03995 (K8W59_03990) 829720..830133 - 414 WP_223397464.1 hypothetical protein -
K8W59_RS04000 (K8W59_03995) 830156..831220 - 1065 WP_223397465.1 DNA recombination protein RmuC -
K8W59_RS04005 (K8W59_04000) 831286..832527 + 1242 WP_223397466.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-116)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-38)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12321.30 Da        Isoelectric Point: 10.6530

>T215583 WP_223399724.1 NZ_CP082922:c827727-827377 [Nocardioides rotundus]
MMLRGEVRLVDLDPARGSESTKRRPAVVVSNDRANAAAARLGRGVVTVVPLTSSVERVFPFQVLLPSEETGLRVDSKAQA
EQVRSVALEGVGPAIGVVPLSLMGQVDAALRLHLQL

Download         Length: 351 bp

>T215583 NZ_CP110816:2609228-2609335 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 7715.41 Da        Isoelectric Point: 3.8705

>AT215583 WP_223397461.1 NZ_CP082922:c827942-827724 [Nocardioides rotundus]
MKLSVSLSEEDVAALDRFVEQTGLDSRSAGIQQAIRRLRGPELAAAYAAAFEEWEDASDAAAWEATTADGLD

Download         Length: 219 bp

>AT215583 NZ_CP110816:c2609181-2609115 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References