Detailed information of TA system TA021557

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 114944..115557
• Replicon: plasmid 3
• Accession: NC_007961
• Organism: Nitrobacter hamburgensis X14

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q1QEX6
• Locus tag: NHAM_RS23745
• Protein ID: WP_011505300.1
• Coordinates: 115246..115557 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q1QEX7
• Locus tag: NHAM_RS23740
• Protein ID: WP_011505299.1
• Coordinates: 114944..115249 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NHAM_RS23710	111168..111644	+	477	WP_011505294.1	hypothetical protein	-
NHAM_RS23715	111641..112510	+	870	WP_011505295.1	hypothetical protein	-
NHAM_RS23720	112507..112794	+	288	Protein_118	hypothetical protein	-
NHAM_RS23725	112913..113213	+	301	Protein_119	hypothetical protein	-
NHAM_RS23730	113605..114195	+	591	WP_011505298.1	hypothetical protein	-
NHAM_RS23735	114272..114631	-	360	Protein_121	tyrosine-type recombinase/integrase	-
NHAM_RS23740	114944..115249	+	306	WP_011505299.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
NHAM_RS23745	115246..115557	+	312	WP_011505300.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NHAM_RS23750	115915..116386	-	472	Protein_124	IS110 family transposase	-
NHAM_RS23760	117229..117630	+	402	WP_041359805.1	single-stranded DNA-binding protein	-
NHAM_RS23765	117712..117969	-	258	WP_011505303.1	WGR domain-containing protein	-
NHAM_RS23770	118053..118406	-	354	WP_198137079.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..121408	121408

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-54)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11505.24 Da
• Isoelectric Point: 6.4689

>T21557 WP_011505300.1 NC_007961:115246-115557 [Nitrobacter hamburgensis X14]
VKLLWSAFALADRDGIFTHFEADNPRAAIVIDERIAAAVRLLVDFPESGRLGRLGRLGRVVGTRELVINDTPYIAAYIVT
ENAIRILRVLHGTQRWPDELPEG

Nucleotide

• Length: 312 bp

>T21557 NC_007961:115246-115557 [Nitrobacter hamburgensis X14]
GTGAAGCTTCTCTGGTCCGCCTTCGCGCTCGCCGATCGCGACGGCATCTTCACCCACTTCGAGGCTGATAACCCTCGCGC
AGCGATCGTGATCGACGAGCGGATCGCCGCCGCGGTGCGCCTCCTCGTCGACTTCCCGGAGAGCGGCCGTCTCGGCCGTC
TCGGCCGTCTCGGCCGTGTCGTCGGCACCCGCGAGCTTGTCATCAACGACACCCCCTATATCGCTGCCTATATCGTGACG
GAGAATGCGATCCGGATTCTGCGCGTTCTGCACGGCACCCAGCGATGGCCCGACGAACTCCCCGAGGGCTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10921.22 Da
• Isoelectric Point: 5.8685

>AT21557 WP_011505299.1 NC_007961:114944-115249 [Nitrobacter hamburgensis X14]
MAANALVQTRIDADVRDRASAVLEGLGLTVSDAVRILLTRTANEGALPLELVTNGEAHDAWFRAKVQEALEDTRPDVSDR
QADAYFASRRAAARKKADAKS

Nucleotide

• Length: 306 bp

>AT21557 NC_007961:114944-115249 [Nitrobacter hamburgensis X14]
ATGGCCGCGAATGCTCTCGTCCAGACCCGTATCGACGCCGACGTGCGGGACCGCGCATCGGCCGTGCTCGAAGGACTGGG
ACTGACCGTTTCAGATGCAGTGCGCATCCTGCTGACGCGGACCGCCAACGAGGGCGCGCTGCCGCTCGAACTGGTGACGA
ACGGCGAGGCGCATGACGCCTGGTTCCGGGCGAAGGTGCAGGAAGCGCTGGAGGACACCCGGCCCGATGTCTCTGATAGG
CAGGCTGACGCATATTTCGCAAGCCGACGCGCAGCGGCGCGGAAAAAGGCCGACGCGAAATCGTGA

Structures

Toxin

• AlphaFold DB: Q1QEX6

Antitoxin

• AlphaFold DB: Q1QEX7