Detailed information of TA system TA021556

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/GNAT-DUF1778
• Location: 40050..40843
• Replicon: plasmid 3
• Accession: NC_007961
• Organism: Nitrobacter hamburgensis X14

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: NHAM_RS23375
• Protein ID: WP_041359829.1
• Coordinates: 40337..40843 (+)
• Length: 169 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: Q1QF38
• Locus tag: NHAM_RS23370
• Protein ID: WP_011505239.1
• Coordinates: 40050..40340 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NHAM_RS25985	35755..36975	+	1221	WP_198137078.1	TniQ family protein	-
NHAM_RS23350	37169..38113	+	945	WP_011505237.1	DUF1403 family protein	-
NHAM_RS23355	38131..38829	+	699	WP_011505238.1	SMC-Scp complex subunit ScpB	-
NHAM_RS23360	39117..39290	-	174	WP_157043864.1	hypothetical protein	-
NHAM_RS25990	39425..39991	+	567	WP_081435108.1	DUF1612 domain-containing protein	-
NHAM_RS23370	40050..40340	+	291	WP_011505239.1	DUF1778 domain-containing protein	Antitoxin
NHAM_RS23375	40337..40843	+	507	WP_041359829.1	GNAT family N-acetyltransferase	Toxin
NHAM_RS23380	41155..42996	+	1842	WP_011505241.1	diguanylate cyclase	-
NHAM_RS23390	43982..45325	+	1344	WP_011510130.1	IS1380-like element ISNha3 family transposase	-
NHAM_RS27370	45569..45823	+	255	WP_157043865.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..121408	121408
	-	flank	IS/Tn	-	-	43982..45325	1343

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-89)

Sequences

Toxin

Protein

• Length: 169 a.a.
• Molecular weight: 18285.13 Da
• Isoelectric Point: 9.5533

>T21556 WP_041359829.1 NC_007961:40337-40843 [Nitrobacter hamburgensis X14]
MTKAAPLSLPEVLREDHATESFDSGEPTLDTWLVRRARANQISGASRTYVVCRERKVVGYYALASGGIDLDAAPGRLRRN
MPDPLPVVVLGRLAIARGEQGQGLGQAMLRDASLRVLQAAERVGVALLVVHALSERAKQFYLSCGFKESPLDPMTLMTRV
KDLGDARV

Nucleotide

• Length: 507 bp

>T21556 NC_007961:40337-40843 [Nitrobacter hamburgensis X14]
CTGACGAAGGCCGCGCCGCTCAGCCTTCCGGAGGTATTGCGGGAGGATCACGCAACCGAAAGCTTCGACAGCGGCGAGCC
GACACTCGATACTTGGCTTGTTCGACGCGCCAGGGCAAACCAGATCAGCGGAGCCTCGCGCACTTACGTCGTCTGCCGGG
AACGAAAGGTCGTCGGATATTACGCGCTCGCCAGCGGCGGGATCGATCTGGACGCGGCGCCAGGCCGGCTCCGGCGTAAC
ATGCCCGATCCGCTTCCCGTCGTGGTCTTGGGCCGCCTTGCCATCGCGCGCGGCGAACAAGGTCAGGGGCTTGGCCAGGC
GATGCTTCGCGACGCCTCCCTGAGAGTGTTGCAGGCGGCCGAGCGCGTTGGCGTGGCCTTGCTCGTGGTCCACGCGCTCT
CTGAACGTGCAAAGCAATTCTACCTCAGTTGCGGATTCAAGGAATCGCCGCTTGATCCCATGACGCTCATGACCAGGGTC
AAAGATCTTGGTGATGCGCGTGTGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10835.43 Da
• Isoelectric Point: 10.5258

>AT21556 WP_011505239.1 NC_007961:40050-40340 [Nitrobacter hamburgensis X14]
MAQARKKDTKSRSLINLRVSPEDRRLIDRAATATGKNRSEFMLNAARFAAEEALLDKVLFRVDAKTYDGLIAYLDEPPSA
DANLRKLMQTTPPWRG

Nucleotide

• Length: 291 bp

>AT21556 NC_007961:40050-40340 [Nitrobacter hamburgensis X14]
ATGGCCCAGGCACGCAAGAAGGACACCAAATCCCGTTCGCTGATCAACCTGCGGGTCTCCCCGGAGGATCGGCGATTGAT
CGACCGCGCCGCCACGGCGACCGGCAAGAACAGGTCGGAATTCATGCTCAATGCCGCGCGGTTTGCGGCCGAGGAGGCGC
TGCTCGACAAGGTGTTATTTCGGGTCGACGCCAAAACCTATGATGGACTGATTGCCTACCTCGACGAGCCGCCGTCCGCG
GATGCAAACCTTCGCAAGCTGATGCAGACCACGCCGCCGTGGCGGGGCTGA

Structures

Antitoxin

• AlphaFold DB: Q1QF38