Detailed information of TA system TA021555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 32241..32881
• Replicon: plasmid 3
• Accession: NC_007961
• Organism: Nitrobacter hamburgensis X14

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q1QF45
• Locus tag: NHAM_RS23325
• Protein ID: WP_011505232.1
• Coordinates: 32241..32627 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q1QF44
• Locus tag: NHAM_RS23330
• Protein ID: WP_011505233.1
• Coordinates: 32627..32881 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NHAM_RS23300	27787..28818	+	1032	WP_011505227.1	plasmid partitioning protein RepB	-
NHAM_RS23305	29095..30420	+	1326	WP_011505228.1	replication initiation protein RepC	-
NHAM_RS23310	30502..30750	+	249	WP_041359822.1	type II toxin-antitoxin system VapB family antitoxin	-
NHAM_RS23315	30747..31160	+	414	WP_011505230.1	type II toxin-antitoxin system VapC family toxin	-
NHAM_RS23320	31165..32238	-	1074	WP_011505231.1	tyrosine-type recombinase/integrase	-
NHAM_RS23325	32241..32627	-	387	WP_011505232.1	type II toxin-antitoxin system VapC family toxin	Toxin
NHAM_RS23330	32627..32881	-	255	WP_011505233.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
NHAM_RS23335	33183..34838	+	1656	WP_011505234.1	DDE-type integrase/transposase/recombinase	-
NHAM_RS23340	34829..35758	+	930	WP_041359759.1	TniB family NTP-binding protein	-
NHAM_RS25985	35755..36975	+	1221	WP_198137078.1	TniQ family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..121408	121408

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13998.87 Da
• Isoelectric Point: 4.9246

>T21555 WP_011505232.1 NC_007961:c32627-32241 [Nitrobacter hamburgensis X14]
MVIDTSPIVAIFFNEPDAARFRERIADDPVRLISAATVLEAAMVIEARLGEAGGTELDLWLHKADVEIVPVTAEHADQAR
RAWRRYGKGRHPAGLNFGDCFSYALAALSGEPLLYKGNDFSQTDIKAA

Nucleotide

• Length: 387 bp

>T21555 NC_007961:c32627-32241 [Nitrobacter hamburgensis X14]
ATGGTGATCGATACGTCCCCGATTGTCGCGATCTTCTTCAATGAGCCGGACGCTGCGCGCTTTCGTGAGCGCATCGCCGA
CGATCCGGTCCGCCTGATCTCGGCGGCAACCGTCCTCGAAGCGGCCATGGTCATCGAGGCCCGTTTGGGCGAGGCGGGAG
GGACAGAACTCGATCTCTGGCTGCATAAGGCGGACGTCGAGATCGTTCCCGTGACTGCGGAGCACGCCGACCAGGCCCGG
CGCGCCTGGCGTCGCTACGGCAAAGGCCGCCACCCGGCCGGATTGAATTTCGGCGACTGCTTTTCCTATGCCCTGGCCGC
CCTCAGCGGTGAACCCCTGCTGTACAAGGGCAACGACTTTTCGCAGACCGACATCAAGGCGGCCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9430.74 Da
• Isoelectric Point: 5.1851

>AT21555 WP_011505233.1 NC_007961:c32881-32627 [Nitrobacter hamburgensis X14]
MALSIKDPETEKLARALAQRTGETITVATRRAIEERLKRTESQARKAALLEDMEAMQRRLNALPILDPRSPDDIIGYDEN
GVPN

Nucleotide

• Length: 255 bp

>AT21555 NC_007961:c32881-32627 [Nitrobacter hamburgensis X14]
ATGGCGCTAAGCATCAAGGATCCCGAGACCGAAAAGCTCGCGCGCGCACTGGCCCAGCGCACCGGGGAGACCATCACCGT
CGCAACCCGCCGCGCGATCGAGGAGCGGCTGAAGCGAACGGAATCGCAGGCGCGCAAGGCCGCCCTTCTGGAGGATATGG
AGGCGATGCAGCGTCGACTGAATGCGCTTCCGATTCTCGATCCCCGCAGCCCCGACGACATCATCGGCTACGACGAAAAT
GGCGTTCCAAACTGA

Structures

Toxin

• AlphaFold DB: Q1QF45

Antitoxin

• AlphaFold DB: Q1QF44