Detailed information of TA system TA215527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 148719..149434
• Replicon: chromosome
• Accession: NZ_CP082909
• Organism: Endozoicomonas sp. 4G

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K7B67_RS00915
• Protein ID: WP_252178488.1
• Coordinates: 148988..149434 (+)
• Length: 149 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K7B67_RS00910
• Protein ID: WP_252178487.1
• Coordinates: 148719..148991 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7B67_RS00870	144075..144599	+	525	WP_252178480.1	molybdenum cofactor biosynthesis protein B	-
K7B67_RS00875	144596..145075	+	480	WP_252180509.1	cyclic pyranopterin monophosphate synthase MoaC	-
K7B67_RS00880	145100..145345	+	246	WP_252178481.1	molybdopterin converting factor subunit 1	-
K7B67_RS00885	145348..145803	+	456	WP_252178482.1	molybdopterin synthase catalytic subunit MoaE	-
K7B67_RS00890	145800..146576	+	777	WP_252178483.1	molybdate ABC transporter substrate-binding protein	-
K7B67_RS00895	146576..147256	+	681	WP_252178484.1	molybdate ABC transporter permease subunit	-
K7B67_RS00900	147267..148340	+	1074	WP_252178485.1	molybdenum ABC transporter ATP-binding protein	-
K7B67_RS00905	148320..148580	-	261	WP_252178486.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
K7B67_RS00910	148719..148991	+	273	WP_252178487.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K7B67_RS00915	148988..149434	+	447	WP_252178488.1	type II toxin-antitoxin system VapC family toxin	Toxin
K7B67_RS00920	149598..151055	-	1458	WP_252178489.1	hypothetical protein	-
K7B67_RS00925	151371..151838	-	468	WP_252178490.1	hypothetical protein	-
K7B67_RS23930	152029..152508	+	480	WP_276576716.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
K7B67_RS00935	152564..154249	-	1686	WP_252178491.1	helix-hairpin-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-68)

Sequences

Toxin

Protein

• Length: 149 a.a.
• Molecular weight: 16929.60 Da
• Isoelectric Point: 5.3029

>T215527 WP_252178488.1 NZ_CP082909:148988-149434 [Endozoicomonas sp. 4G]
VKYLLDTCVLSEAFKKNPDKHVLEWIDSREENRLYLSVLTLAEIEKGIIKCQEPVRQEKLRTWLESDLMERFKHRILPID
LGVAIAWGRIQAESEKKGTPMPVIDGLIAVTGLVNDCTVVTRNTGDMRMSQVQLYNPWEDTAPFCGVN

Nucleotide

• Length: 447 bp

>T215527 NZ_CP110809:c4299226-4299044 [Pseudomonas syringae]
GTGCAAAGCAGGCAATTGATAAAGGAGCTTGAAGCTGATGGATGGGTTCTTGATCGTGTTTCGGGCAGTCACCATATGTT
CAAGCATCCTGAAAAGCTTCAGACAGTCCCCGTGCCGCATCCCAAGAAAGACTTGCCATTCGGAACGGTGCGGGCCATCA
AAAAGCTTGCCGGGCTGGTTTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10357.49 Da
• Isoelectric Point: 5.5623

>AT215527 WP_252178487.1 NZ_CP082909:148719-148991 [Endozoicomonas sp. 4G]
MTEGISMENQWQLQEAKSKFSHIVDRARQHGPQFVTRHGHKAAVVLSFEDYQRMCAPENSLSEFLKASPFSDSDVETFDR
SKETPRDVDL

Nucleotide

• Length: 273 bp

>AT215527 NZ_CP110809:c4299007-4298603 [Pseudomonas syringae]
ATGAAATACCCAATGTGCATAGAATGGGGAAACGAGACCACGGCGTTTGGTATCCAGATTCCCGATATTCCCGGTGCCGT
AACAGCTGGCGATACGTTCGAGGAAGCCCACGCTGCGGCGGTTGAGATTGCTCATATCATGCTGCAGGAGATAGCAGCCA
GCGGTAGAACCATACCCAAGGCCGGAAACGTTGCCGAGCACGCCAGGAATCCCGATTTCGCAGGGATGGGCTGGGGCATG
ATCGAGATTGACGTAACGCCTTATCTCGGCAAGACGGAGAAGGTCAACGTCACACTGCCTGGTTTCGTGATCAGGCAGAT
TGATCGCTATGTGCGGGATCACAGCATCAAGAGCCGCTCGACCTTTCTGGCCGACGCGGCGCTGGAAAAACTGGGTCGAG
CATGA