Detailed information of TA system TA215525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2630882..2631472
• Replicon: chromosome
• Accession: NZ_CP082904
• Organism: Mixta hanseatica isolate X22927

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: K6958_RS12695
• Protein ID: WP_249891441.1
• Coordinates: 2630882..2631214 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: K6958_RS12700
• Protein ID: WP_249891442.1
• Coordinates: 2631215..2631472 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6958_RS12675 (K6958_12675)	2627219..2628121	+	903	WP_249891438.1	LysR substrate-binding domain-containing protein	-
K6958_RS12680 (K6958_12680)	2628206..2628703	+	498	WP_249891439.1	pyridoxamine 5'-phosphate oxidase family protein	-
K6958_RS12685 (K6958_12685)	2628771..2630219	-	1449	WP_249891440.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
K6958_RS12695 (K6958_12695)	2630882..2631214	-	333	WP_249891441.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K6958_RS12700 (K6958_12700)	2631215..2631472	-	258	WP_249891442.1	antitoxin	Antitoxin
K6958_RS12705	2631820..2631999	-	180	WP_249891443.1	helix-turn-helix domain-containing protein	-
K6958_RS12710 (K6958_12710)	2632065..2632325	+	261	Protein_2489	IS3 family transposase	-
K6958_RS12715 (K6958_12715)	2632384..2632815	+	432	WP_249891444.1	DUF1795 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11836.66 Da
• Isoelectric Point: 10.1839

>T215525 WP_249891441.1 NZ_CP082904:c2631214-2630882 [Mixta hanseatica]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTNGGNFARAAGFTVSLDDAGTKTTGVIRCDQPRTI
DMGARNGKRLERIPDAVVNEVLARLEAILS

Nucleotide

• Length: 333 bp

>T215525 NZ_CP110809:c1111223-1110819 [Pseudomonas syringae]
ATGCTCAAGTACATGCTTGACACCAATATTTGCATTTTCACTATAAAGAACAAGCCAACTTCGGTGCGTGAAGCCTTCAA
CCTCCATCATGGACAGCTATGTATCAGCGCGATCACGTTGATGGAGCTTGTCTACGGCGCAGAAAAGTCTTTGAGCCCCG
AAAGAAACCTTGCCGTTGTAGAGGGTTTCACAGCGCGTCTTGAAGTGCTCCCTTACGACAGCGACGCTGCGGCTCATACC
GGCATGATCAGAGCCGAACTGGCTAGATCGGGCACACCGATCGGCCCGTACGATCAAATGATTGCAGGACACGCCCGCTC
TCTAGGCCTGGTGGTGATCACTAATAATCAGCGTGAATTCCGACGCGTCGAGGGGCTACGAGTAGAAGACTGGGTAAGCC
AGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9441.93 Da
• Isoelectric Point: 4.4620

>AT215525 WP_249891442.1 NZ_CP082904:c2631472-2631215 [Mixta hanseatica]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWINAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT215525 NZ_CP110809:c1111453-1111223 [Pseudomonas syringae]
ATGGAACAATCCACAGTCTTCAAAAGCAATCGAAGCCAGGCTGTCCGACTGCCAAAAGCGGTCGCCTTGCCAGACGACGT
GAAACGTGTCGATGTCGTAGCCGTGGGCAGAACCCGCATTATTTCCCCCGCCGGTGAAATGTGGAACAGCTGGTTCGATG
GTGAAAGTGTCAGTGATGATTTCATGGCAGAGCGTGAACAACCAGCCGAACAGCAGCGCGAATCGCTCTGA