Detailed information of TA system TA215515

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1162160..1162737
• Replicon: chromosome
• Accession: NZ_CP082904
• Organism: Mixta hanseatica isolate X22927

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K6958_RS05515
• Protein ID: WP_249893720.1
• Coordinates: 1162160..1162492 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: K6958_RS05520
• Protein ID: WP_249893721.1
• Coordinates: 1162486..1162737 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6958_RS05510 (K6958_05525)	1161757..1162134	+	378	WP_249893719.1	hypothetical protein	-
K6958_RS05515 (K6958_05530)	1162160..1162492	-	333	WP_249893720.1	endoribonuclease MazF	Toxin
K6958_RS05520 (K6958_05535)	1162486..1162737	-	252	WP_249893721.1	antitoxin MazE	Antitoxin
K6958_RS05525 (K6958_05540)	1163006..1163215	+	210	WP_249893722.1	DUF1471 domain-containing protein	-
K6958_RS05530 (K6958_05545)	1163586..1164617	+	1032	WP_249893723.1	cell division protein ZapE	-
K6958_RS05535 (K6958_05550)	1164788..1165645	+	858	WP_249893724.1	glucose 1-dehydrogenase	-
K6958_RS05540 (K6958_05555)	1165693..1166514	-	822	WP_249893725.1	Cof-type HAD-IIB family hydrolase	-
K6958_RS05545 (K6958_05560)	1166698..1167087	-	390	WP_249893726.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-101)

Antitoxin

• Antitoxin PemI-like (1-83)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12147.04 Da
• Isoelectric Point: 8.0519

>T215515 WP_249893720.1 NZ_CP082904:c1162492-1162160 [Mixta hanseatica]
MVAKYVPDAGDVVWLHFDPQAGHEQAGHRPALVLSAKQYNEMVGLMVWCPMTTKIKGYPFEVQLDSQPGSAVLADQVKSL
DWRAREATKKGRVTAAELESVRVISRLLVG

Nucleotide

• Length: 333 bp

>T215515 NZ_CP110806:c3729500-3729195 [Cellulomonas sp. S1-8]
ATGCGCCCGATCCACGTCGCGCAGCTCGACAAGGCGCGGCCCGTCCTCGTCCTCACGCGGGAGCTGGTGCGCCCGCACCT
GTCGAACGTCACCGTCGCCCCGATCACGAGCACGATCCGCGGGCTGTCGACCGAGGTGCCGCTGGGGCCGGTCAACGGTC
TCGACCACGACTGCGTCGCGAGCTGCGACAACCTGCAGACCGTGCCGGTGTCCGCGCTGGGACGGCTGGTGGGGTACCTG
CTGCCGGACCAGGAGGCGCGCCTCGCGCTGGCGATCGCGCTGGCGTTCGACCTGGAGCCGGGGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9097.39 Da
• Isoelectric Point: 4.3611

>AT215515 WP_249893721.1 NZ_CP082904:c1162737-1162486 [Mixta hanseatica]
MRQTIKRWGNSPAVRIPSQLMAAMALDVDAEVEVNVVFGSDNRPQLVIEPVTPAPTLEALLAKVTPENIHSEAFTDEVGK
EAW

Nucleotide

• Length: 252 bp

>AT215515 NZ_CP110806:c3729742-3729500 [Cellulomonas sp. S1-8]
ATGACGATCCAGATCACCGTGCGGCTCGACGACGACCTCGTCGAGGCCGTCGACGCGCTCGTCGCCTCCGGGAGGGCGCG
CAGCCGCGCGTCGGTCGTCGAGGTCGCCCTCGAGCGACACCTGCGCCGGGAGCTGGCCGCGCGCGACGTCGAGATCCTGC
GCGGGGACGTGGCGTCCGGCGGTGACCTCGACGGGCTCGCCGCGTGGGCCCAGCAGCTGCCCGCGTCGGCGCAGCTCGAC
TGA