Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3968078..3968697 Replicon chromosome
Accession NZ_CP082898
Organism Klebsiella aerogenes strain KAE3SP

Toxin (Protein)


Gene name Hha Uniprot ID A0A0H3FXE2
Locus tag K6006_RS18740 Protein ID WP_015367918.1
Coordinates 3968479..3968697 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0H3FPM3
Locus tag K6006_RS18735 Protein ID WP_015367917.1
Coordinates 3968078..3968452 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6006_RS18725 (K6006_18695) 3963237..3964436 + 1200 WP_020079451.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K6006_RS18730 (K6006_18700) 3964459..3967605 + 3147 WP_015704610.1 multidrug efflux RND transporter permease subunit AcrB -
K6006_RS18735 (K6006_18705) 3968078..3968452 + 375 WP_015367917.1 Hha toxicity modulator TomB Antitoxin
K6006_RS18740 (K6006_18710) 3968479..3968697 + 219 WP_015367918.1 HHA domain-containing protein Toxin
K6006_RS18745 (K6006_18715) 3968829..3969392 + 564 WP_015704609.1 maltose O-acetyltransferase -
K6006_RS18750 3969367..3969471 - 105 Protein_3674 hypothetical protein -
K6006_RS18755 (K6006_18720) 3969518..3969982 + 465 WP_015367920.1 YlaC family protein -
K6006_RS18760 (K6006_18725) 3969957..3971411 - 1455 WP_221696158.1 PLP-dependent aminotransferase family protein -
K6006_RS18765 (K6006_18730) 3971514..3972224 + 711 WP_015704607.1 GNAT family protein -
K6006_RS18770 (K6006_18735) 3972221..3972361 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
K6006_RS18775 (K6006_18740) 3972364..3972624 - 261 WP_015367923.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8569.96 Da        Isoelectric Point: 9.4828

>T215514 WP_015367918.1 NZ_CP082898:3968479-3968697 [Klebsiella aerogenes]
MSGKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T215514 NZ_CP110806:3674108-3674539 [Cellulomonas sp. S1-8]
GTGATCCTGCCGGACGTCAACGTCCTCGTCGGGGCCTTCCGTGCCGACGCCGCGCGGCACGACGCGTTGCGCACCTGGCT
CGAGGCCGCGGTGGAGGCACCCGAGCCGCTCGGCCTCACCGACGCGGTGCTCGGCGGCACCGTGCGCGTCCTGACGCACC
CCCGGGTCTTCGTCCGGCCCAGCCCGCTGCGCACCGCGCTCGACCTCGTCGAGCAGCTGCGCTCGCACCCCGGGACCGTG
CGCGTGGCACCAGGCTCGCGGCACTGGGAGCTGGTCGCCGACCTGTGCCGGGCCGCCGACGCCCGCGGCAACCTCGTGGC
CGACGCCCAGCACGCCGCCGTGGCGGTCGAGCAGGGCGCGACGTGGGTCTCGCAGGACCGCGACTTCGCGCGCTTCCCCG
GTCTGCGTTGGAAGGTCGCCGTCGAGGCATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14464.21 Da        Isoelectric Point: 4.9045

>AT215514 WP_015367917.1 NZ_CP082898:3968078-3968452 [Klebsiella aerogenes]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNRGWVNDPTSAVNLQLNELIEHIATYALNYKIKYAEDNKLISQLDEYL
DDTFMLFSSYGINTSDLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT215514 NZ_CP110806:3673869-3674111 [Cellulomonas sp. S1-8]
ATGCGAACAACGCTGCACCTGCCGGACGCCCTGTACCGGGACGTCAAGGCCGCCGCCGCGCAGTCCGGTCGGACGGTCAC
CTCCGTCGTCGAGGACGCGCTGCGGGCCGAGCTCGCCCGGTTCCGTGCCGGCACCACCGACCGCACACCGTTCCGCATCG
ACGCCGTCGGCACGGGCGGCGTGCTGGCGGGCGTCGACCTGGACGACTCCGCCGCGCTCCTCGACCTGGTGGAGGGCCGG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3FXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3FPM3

References