Detailed information of TA system TA215513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2688053..2688792
• Replicon: chromosome
• Accession: NZ_CP082898
• Organism: Klebsiella aerogenes strain KAE3SP

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: K6006_RS12705
• Protein ID: WP_032711618.1
• Coordinates: 2688307..2688792 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A0H3FSM9
• Locus tag: K6006_RS12700
• Protein ID: WP_015705366.1
• Coordinates: 2688053..2688319 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6006_RS12675 (K6006_12655)	2683388..2683975	+	588	WP_221696111.1	hypothetical protein	-
K6006_RS12680 (K6006_12660)	2683972..2685291	+	1320	WP_042897499.1	ATP-binding protein	-
K6006_RS12685 (K6006_12665)	2685291..2685908	+	618	WP_032705773.1	response regulator	-
K6006_RS12690 (K6006_12670)	2686004..2686501	+	498	WP_032711620.1	heme-binding protein	-
K6006_RS12695 (K6006_12675)	2686545..2687780	-	1236	WP_032711619.1	MFS transporter	-
K6006_RS12700 (K6006_12680)	2688053..2688319	+	267	WP_015705366.1	DUF1778 domain-containing protein	Antitoxin
K6006_RS12705 (K6006_12685)	2688307..2688792	+	486	WP_032711618.1	GNAT family N-acetyltransferase	Toxin
K6006_RS12710 (K6006_12690)	2688864..2690480	+	1617	WP_047054692.1	FAD-NAD(P)-binding protein	-
K6006_RS12715 (K6006_12695)	2690599..2691699	+	1101	WP_015366663.1	NADH:flavin oxidoreductase/NADH oxidase	-
K6006_RS12720 (K6006_12700)	2691756..2691914	-	159	WP_002903230.1	YqaE/Pmp3 family membrane protein	-
K6006_RS12725 (K6006_12705)	2692175..2693245	+	1071	WP_020078888.1	mannonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17605.23 Da
• Isoelectric Point: 8.8590

>T215513 WP_032711618.1 NZ_CP082898:2688307-2688792 [Klebsiella aerogenes]
MGKVSAPTPLSSHHQIAEFCCGETVLDQWLKQRGLKNQAQGAARTFVVCKEDSHQVVGFYSLATGSVNHTEATGGLRRNM
PDPIPVIILARLAIDCAYHGQGLGADLLHDALLRSYRVAENVGVRALMVHALTDSAKRFYLHHGFKASTTQERTLFLALP
K

Nucleotide

• Length: 486 bp

>T215513 NZ_CP110806:c648329-647928 [Cellulomonas sp. S1-8]
GTGATCGTCGACACCTCGGCCGTCGTCGCGATCCTCCTGGGAGAGCCGGGTGCCGACGAGCTCGTTCGCTCGGTCCTGGC
CGACCCGCGCCCGCGCATGTCAGCGCCGACGTTCGTCGAGCTCTCGGCGGTCGTGGCCCAGAAGGGCGCGCCCCAGCAGC
GCCGGCGCGTCGAGTCCCTGCTCGAGCGGCTCGGCATCGAGGTGGTCGCGATGACGCCCGAGCACGCCCGGATCGCGGCC
GACGCGTACCGCGAGCTCGGCCGTGGCAGCGGGCACCCCGCCCGCCTGAACCTCGGCGACTGCTTCTCCTACGCCCTGGC
CACCGAGCAGGACGAGCCGCTGCTGTTCGTCGGCGACGACTTCTCCCACACGGACCTGCGTCCCGCCCGGGCACGGGACT
GA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10085.56 Da
• Isoelectric Point: 4.6558

>AT215513 WP_015705366.1 NZ_CP082898:2688053-2688319 [Klebsiella aerogenes]
MKSDIQLNIRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNLSDEQYAEFIDMLDAPVEANPALDKLL
ARKPQWEK

Nucleotide

• Length: 267 bp

>AT215513 NZ_CP110806:c648598-648326 [Cellulomonas sp. S1-8]
ATGTCGCTCAACATCAAGAACGAGCGGACGCACGCCCTCGTCCGCCGGCTCGCCGAGCTGACGGGGCAGTCTCAGACCGC
CGCCGTGGAGGACGCGGTCGCGCGCCGCCTCGCGGAGGTCGAGTCGCTCGCCCCGGTCGGGCGGGTCGACACGCGGTGGG
CCGACGTGCAGCGGCTCCTCGTCGACTACCGGGCCGACCTGAGCCCGCAGGACCGCGCCCGGATCGCGGACGCGGACTCC
GCGCTCTACGACGACCTCGGCCTCCCCAGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3FSM9