Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 271137..271768 Replicon chromosome
Accession NZ_CP082898
Organism Klebsiella aerogenes strain KAE3SP

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H3FL31
Locus tag K6006_RS01235 Protein ID WP_015703741.1
Coordinates 271137..271412 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0H3FNK6
Locus tag K6006_RS01240 Protein ID WP_015703740.1
Coordinates 271409..271768 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6006_RS01215 (K6006_01215) 266366..266701 + 336 WP_015703743.1 universal stress protein UspB -
K6006_RS01220 (K6006_01220) 266766..268262 - 1497 WP_015369272.1 inorganic phosphate transporter PitA -
K6006_RS01225 (K6006_01225) 268493..269686 + 1194 WP_015703742.1 NAD(P)/FAD-dependent oxidoreductase -
K6006_RS01230 (K6006_01230) 269727..270740 - 1014 WP_015369274.1 magnesium transporter -
K6006_RS01235 (K6006_01235) 271137..271412 + 276 WP_015703741.1 type II toxin-antitoxin system HicA family toxin Toxin
K6006_RS01240 (K6006_01240) 271409..271768 + 360 WP_015703740.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
K6006_RS01245 (K6006_01245) 271796..272197 - 402 WP_020077744.1 nickel-responsive transcriptional regulator NikR -
K6006_RS01250 (K6006_01250) 272185..272976 - 792 WP_086630592.1 nickel import ATP-binding protein NikE -
K6006_RS01255 (K6006_01255) 272973..273737 - 765 WP_221696185.1 nickel import ATP-binding protein NikD -
K6006_RS01260 (K6006_01260) 273737..274570 - 834 WP_015369278.1 nickel ABC transporter permease subunit NikC -
K6006_RS01265 (K6006_01265) 274567..275511 - 945 WP_045386598.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10258.89 Da        Isoelectric Point: 10.4888

>T215502 WP_015703741.1 NZ_CP082898:271137-271412 [Klebsiella aerogenes]
MEQQVLSLRKKQRNTLEQLFKTPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

>T215502 NZ_CP110791:1265466-1265753 [Yersinia sp. SCPM-O-B-9106 (C-191)]
ATGATCTATACCGTTAAGTTCAGAGAAGAAGCGCTAAAGGAATGGCACAAACTGGATAAGACGATACAACAGCAGTTCGC
CAAAAAATTAAAAAAGTGCAGCGATAATCCACATATTGAGAGTGCTAAACTACGTGGGATGAAAGACTGTTACAAAATTA
AATTACGGTCCTCAGGTTTCCGACTAGTGTATGAAGTTATTGATGATATTTTGATAGTTGCTGTGGTTGCAGTAGGAAAA
AGAGAACGTAGCGAGGTTTATAAACTTGCCAGTGAAAGATTACGCTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13305.02 Da        Isoelectric Point: 4.3472

>AT215502 WP_015703740.1 NZ_CP082898:271409-271768 [Klebsiella aerogenes]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCREAGIEPYAQPEKLKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp

>AT215502 NZ_CP110791:1265218-1265469 [Yersinia sp. SCPM-O-B-9106 (C-191)]
ATGCCAAGTGTAATTTTGTCAGACACTAGCGCGAGTGTGAGTGAACTGAAGAAAAATCCCATGGCGACAGTGAATGCCGG
AGCTGGTTTCCCTGTTGCTATCCTGAACCGTAATCAACCTGCGTTTTATTGTGTGCCTGCTGAACTTTATGAACAAATAA
TGGAAGCGCTTGACGATAATGAATTGGCTAAGTTGGTTAACGAACGTCGTAACCAACCACTTCATGATATTGATTTGGAA
AGCTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3FL31

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3FNK6

References