Detailed information of TA system TA215488

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/Fic(toxin)
• Location: 5263..6181
• Replicon: plasmid unnamed3
• Accession: NZ_CP082880
• Organism: Campylobacter coli strain FDAARGOS_1465

Toxin (Protein)

• Gene name: Fic
• Uniprot ID: A0A3Z8EYW1
• Locus tag: K8O90_RS01235
• Protein ID: WP_002790624.1
• Coordinates: 5263..5946 (-)
• Length: 228 a.a.

Antitoxin (Protein)

• Gene name: Fti
• Uniprot ID: -
• Locus tag: K8O90_RS01240
• Protein ID: WP_002779838.1
• Coordinates: 5939..6181 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8O90_RS01205 (K8O90_01180)	583..966	+	384	WP_002797386.1	DNA transfer protein	-
K8O90_RS01210 (K8O90_01185)	929..3085	+	2157	WP_002838514.1	relaxase/mobilization nuclease domain-containing protein	-
K8O90_RS01215 (K8O90_01190)	3326..3550	-	225	WP_002790621.1	hypothetical protein	-
K8O90_RS01220 (K8O90_01195)	3553..4239	-	687	WP_002791558.1	AAA family ATPase	-
K8O90_RS01225 (K8O90_01200)	4331..4726	-	396	WP_002782959.1	conjugal transfer protein TraM	-
K8O90_RS01230 (K8O90_01205)	4791..5258	-	468	WP_002798254.1	signal peptidase I	-
K8O90_RS01235 (K8O90_01210)	5263..5946	-	684	WP_002790624.1	Fic family protein	Toxin
K8O90_RS01240 (K8O90_01215)	5939..6181	-	243	WP_002779838.1	hypothetical protein	Antitoxin
K8O90_RS01245 (K8O90_01220)	6272..6991	-	720	WP_002784845.1	conjugal transfer protein TraL	-
K8O90_RS01250 (K8O90_01225)	7002..8825	-	1824	WP_215447248.1	type IV secretory system conjugative DNA transfer family protein	-
K8O90_RS01255 (K8O90_01230)	8822..11035	-	2214	WP_002795783.1	zincin-like metallopeptidase domain-containing protein	-
K8O90_RS01260 (K8O90_01235)	11040..11180	-	141	WP_002779802.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..25043	25043

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (60-169)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 228 a.a.
• Molecular weight: 25802.76 Da
• Isoelectric Point: 10.0206

>T215488 WP_002790624.1 NZ_CP082880:c5946-5263 [Campylobacter coli]
MIDKVLKFLDDGIPKNDFDRQLLASNLVGATNLIELNKKERIITLQAAKKLELNPIKGNFDYQHLKDIHKALFKDVYTWA
GKDRMEVGLHGNFGKYAPNGTITNFVPGKDLNVTAQQIFTWLKEDNYLTNSKDLNDFAKNLAEFARNLNALHPFREGNGR
TQRIFLNELAKNAGYKLDLNLIPKDKMIMASVEASQLKLGKLEAIIKTNLKSFRQNLDLEQNKGISL

Nucleotide

• Length: 684 bp

>T215488 NZ_CP110789:1992824-1992907 [Yersinia intermedia]
ATTAATGCCAACTTTTAGCGCACGGCTCTGTCCCAAGAGCCATTTCCCTAGACCGAATATAGGAATCGTATTCGGTCTTT
TTTT

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9478.72 Da
• Isoelectric Point: 4.2508

>AT215488 WP_002779838.1 NZ_CP082880:c6181-5939 [Campylobacter coli]
MQKQYPEVYSLEESLAILKKYKDDLTKEQYEQNKSIICGFAIENMFANEEDIINLIKVDKQEKTPDEIIAEYKKEWGVND

Nucleotide

• Length: 243 bp

>AT215488 NZ_CP110789:c1992915-1992824 [Yersinia intermedia]
AACAAGTTAAAAAAAGACCGAATACGATTCCTATATTCGGTCTAGGGAAATGGCTCTTGGGACAGAGCCGTGCGCTAAAA
GTTGGCATTAAT

Structures

Toxin

• AlphaFold DB: A0A3Z8EYW1