Detailed information of TA system TA021548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 2407409..2408013
• Replicon: chromosome
• Accession: NC_007952
• Organism: Burkholderia xenovorans LB400

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BXE_RS32635
• Protein ID: WP_193352970.1
• Coordinates: 2407409..2407714 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q13LE6
• Locus tag: BXE_RS32640
• Protein ID: WP_011492395.1
• Coordinates: 2407711..2408013 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BXE_RS32595	2402650..2403165	-	516	WP_049782302.1	Lrp/AsnC ligand binding domain-containing protein	-
BXE_RS32605	2404037..2404309	-	273	WP_011492388.1	hypothetical protein	-
BXE_RS32610	2404374..2404832	-	459	WP_011492389.1	DoxX family protein	-
BXE_RS32615	2405044..2405451	-	408	WP_011492390.1	VOC family protein	-
BXE_RS32620	2405610..2406074	-	465	WP_011492391.1	transporter RbsD/FucU	-
BXE_RS32625	2406357..2406590	-	234	Protein_2120	hypothetical protein	-
BXE_RS32630	2406616..2406984	-	369	WP_011492393.1	nuclear transport factor 2 family protein	-
BXE_RS32635	2407409..2407714	+	306	WP_193352970.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BXE_RS32640	2407711..2408013	+	303	WP_011492395.1	putative addiction module antidote protein	Antitoxin
BXE_RS32645	2408149..2409051	+	903	WP_081435596.1	helix-turn-helix transcriptional regulator	-
BXE_RS32650	2409126..2409659	+	534	WP_011492397.1	GNAT family N-acetyltransferase	-
BXE_RS32655	2410295..2410615	+	321	WP_007177882.1	flagellar transcriptional regulator FlhD	-
BXE_RS32660	2410630..2411199	+	570	WP_011492398.1	flagellar transcriptional regulator FlhC	-
BXE_RS32665	2411352..2412680	-	1329	WP_040124527.1	Nramp family divalent metal transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-94)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11763.46 Da
• Isoelectric Point: 8.8770

>T21548 WP_193352970.1 NC_007952:2407409-2407714 [Paraburkholderia xenovorans LB400]
VYTVNRTEEFDAWLARLSDQRARAKILVRIRRAERGHFGDMKLLEDGVSEMRIDYGPGYRVYFAREERVVYLLLCGGDKS
TQPADIKHAKTMWAAIREELS

Nucleotide

• Length: 306 bp

>T21548 NC_007952:2407409-2407714 [Paraburkholderia xenovorans LB400]
GTGTACACGGTCAACCGCACCGAGGAGTTCGACGCCTGGCTCGCGCGGCTCTCCGATCAAAGAGCGAGGGCGAAAATACT
CGTGCGAATCCGGCGTGCGGAACGCGGGCATTTCGGCGACATGAAGTTGCTGGAAGACGGTGTGTCAGAGATGCGCATCG
ACTACGGCCCGGGTTATCGGGTCTACTTCGCGCGTGAAGAACGCGTGGTCTATCTGCTGCTCTGCGGTGGCGACAAGTCC
ACCCAGCCAGCCGATATCAAGCATGCCAAAACAATGTGGGCAGCAATCAGAGAGGAACTGTCATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10545.22 Da
• Isoelectric Point: 5.8505

>AT21548 WP_011492395.1 NC_007952:2407711-2408013 [Paraburkholderia xenovorans LB400]
MSKIKTARFDASHYLDSEEMIAEYLNAALEEGDADLLLAAIADIAKARGIAKIAADAGLGRESLYKTLAPGSKPRMDTVF
KLLRALGVKLNAVPEGVAAA

Nucleotide

• Length: 303 bp

>AT21548 NC_007952:2407711-2408013 [Paraburkholderia xenovorans LB400]
ATGAGCAAGATCAAAACCGCACGGTTCGACGCGTCACACTACCTCGATAGCGAGGAAATGATCGCCGAGTATCTCAACGC
CGCGCTGGAAGAAGGCGACGCCGACCTCCTGCTCGCCGCAATCGCCGACATCGCCAAAGCGCGCGGCATCGCCAAGATCG
CGGCAGATGCCGGACTTGGCCGCGAAAGCCTCTACAAAACGCTCGCGCCCGGTTCCAAACCACGCATGGATACGGTGTTC
AAGCTGCTGCGCGCGCTCGGCGTCAAACTCAACGCGGTGCCGGAAGGCGTGGCGGCGGCCTGA

Structures

Antitoxin

• AlphaFold DB: Q13LE6