Detailed information of TA system TA215470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1835282..1835913
• Replicon: chromosome
• Accession: NZ_CP082857
• Organism: Haemophilus aegyptius strain FDAARGOS_1478

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q4QLW0
• Locus tag: K8O83_RS09460
• Protein ID: WP_005651560.1
• Coordinates: 1835515..1835913 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q4QLV9
• Locus tag: K8O83_RS09455
• Protein ID: WP_005648011.1
• Coordinates: 1835282..1835515 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8O83_RS09430 (K8O83_09320)	1830761..1831963	-	1203	WP_006995609.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
K8O83_RS09435 (K8O83_09325)	1832127..1832792	+	666	WP_013527272.1	DNA repair protein RadC	-
K8O83_RS09440 (K8O83_09330)	1833006..1833242	+	237	WP_006995608.1	50S ribosomal protein L28	-
K8O83_RS09445 (K8O83_09335)	1833254..1833424	+	171	WP_005613503.1	50S ribosomal protein L33	-
K8O83_RS09450 (K8O83_09340)	1833771..1835135	+	1365	WP_006995606.1	diaminobutyrate--2-oxoglutarate transaminase	-
K8O83_RS09455 (K8O83_09345)	1835282..1835515	+	234	WP_005648011.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
K8O83_RS09460 (K8O83_09350)	1835515..1835913	+	399	WP_005651560.1	tRNA(fMet)-specific endonuclease VapC	Toxin
K8O83_RS09465 (K8O83_09355)	1835933..1837468	+	1536	WP_006995605.1	L-2,4-diaminobutyrate decarboxylase	-
K8O83_RS09470 (K8O83_09360)	1837711..1838526	+	816	WP_005648023.1	DNA-formamidopyrimidine glycosylase	-
K8O83_RS09475 (K8O83_09365)	1838600..1839622	-	1023	WP_065246977.1	outer membrane-stress sensor serine endopeptidase DegS	-
K8O83_RS09480 (K8O83_09370)	1839623..1840741	-	1119	WP_006995603.1	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15115.46 Da
• Isoelectric Point: 8.0514

>T215470 WP_005651560.1 NZ_CP082857:1835515-1835913 [Haemophilus aegyptius]
VLKYMLDTNIVIYVIKRRPLEILSRFNQNAGKMCVSSITVAELYYGAEKSEYPERNIAVIEDFLSRLTILDYQPKHAAHF
GNIKAELSKQGKLIGENDIHIAAHARSEGLILVSNNLREFERVIALRTENWV

Nucleotide

• Length: 399 bp

>T215470 NZ_CP110778:c32589-32173 [Citrobacter freundii]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8880.98 Da
• Isoelectric Point: 4.4013

>AT215470 WP_005648011.1 NZ_CP082857:1835282-1835515 [Haemophilus aegyptius]
MIEASVFMTNRSQAVRLPAEVRFSEEIKKLSVRVSGSDRILSPLNQSWDSFFLNDQAVSDDFMNEREIAFQPEREAL

Nucleotide

• Length: 234 bp

>AT215470 NZ_CP110778:c32816-32586 [Citrobacter freundii]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: Q4QLW0

Antitoxin

• AlphaFold DB: A0A806DGS0