Detailed information of TA system TA215464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-DUF971
• Location: 1772270..1772859
• Replicon: chromosome
• Accession: NZ_CP082856
• Organism: Haemophilus influenzae strain FDAARGOS_1479

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A852PKG5
• Locus tag: K8P04_RS08750
• Protein ID: WP_005662099.1
• Coordinates: 1772270..1772569 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: K8P04_RS08755
• Protein ID: WP_015702300.1
• Coordinates: 1772566..1772859 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8P04_RS08705 (K8P04_08625)	1768279..1768791	-	513	WP_011272619.1	terminase small subunit	-
K8P04_RS08710 (K8P04_08630)	1768868..1769119	+	252	WP_015702292.1	type II toxin-antitoxin system RelE/ParE family toxin	-
K8P04_RS08715 (K8P04_08635)	1769103..1769450	+	348	WP_015702293.1	helix-turn-helix transcriptional regulator	-
K8P04_RS08720 (K8P04_08640)	1769452..1769733	-	282	WP_041175081.1	hypothetical protein	-
K8P04_RS08725 (K8P04_08645)	1769645..1769968	-	324	WP_041175082.1	DUF2570 family protein	-
K8P04_RS08730 (K8P04_08650)	1769961..1770564	-	604	Protein_1662	glycoside hydrolase family 19 protein	-
K8P04_RS08735 (K8P04_08655)	1770533..1770889	-	357	WP_005650535.1	phage holin, lambda family	-
K8P04_RS08740 (K8P04_08660)	1771013..1771150	-	138	WP_015702297.1	hypothetical protein	-
K8P04_RS08745 (K8P04_08665)	1771225..1771902	-	678	WP_015702298.1	BRO family protein	-
K8P04_RS08750 (K8P04_08670)	1772270..1772569	+	300	WP_005662099.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K8P04_RS08755 (K8P04_08675)	1772566..1772859	+	294	WP_015702300.1	putative addiction module antidote protein	Antitoxin
K8P04_RS08760 (K8P04_08680)	1772891..1773259	-	369	WP_015702301.1	antiterminator Q family protein	-
K8P04_RS08765 (K8P04_08685)	1773261..1773815	-	555	WP_077643895.1	recombination protein NinG	-
K8P04_RS08770	1773902..1774021	-	120	Protein_1670	elongation factor Tu	-
K8P04_RS08775 (K8P04_08695)	1774101..1774517	-	417	WP_005662137.1	recombination protein NinB	-
K8P04_RS08780 (K8P04_08700)	1774554..1774778	-	225	WP_015702303.1	hypothetical protein	-
K8P04_RS08785 (K8P04_08705)	1774775..1775401	-	627	WP_015702304.1	replication protein P	-
K8P04_RS08790 (K8P04_08710)	1775398..1776120	-	723	WP_077643894.1	helix-turn-helix domain-containing protein	-
K8P04_RS08795 (K8P04_08715)	1776117..1776785	-	669	WP_041175131.1	phage regulatory protein/antirepressor Ant	-
K8P04_RS08800 (K8P04_08720)	1776777..1777082	+	306	Protein_1676	phage antirepressor KilAC domain-containing protein	-
K8P04_RS08805 (K8P04_08725)	1777079..1777240	+	162	Protein_1677	helix-turn-helix domain-containing protein	-
K8P04_RS08810 (K8P04_08730)	1777299..1777595	-	297	WP_015702309.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1748460..1801064	52604

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-86)

Antitoxin

• Cro/C1-type helix-turn-helix domain (46-89)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11197.10 Da
• Isoelectric Point: 10.4328

>T215464 WP_005662099.1 NZ_CP082856:1772270-1772569 [Haemophilus influenzae]
MTIQIKTTLTFDSWLSKLKNLRAKAKINARIKRLQFGNFGDIKSVNDGIFELRIDEGQGYRIYLKNQNGVLVILLCGGDK
STQEKDIKQAKLLAQELGL

Nucleotide

• Length: 300 bp

>T215464 NZ_CP110775:3613294-3613653 [Citrobacter freundii]
ATGACGAAACCACTTTACTGGGTTGGGCAGGCCAGAAAAGACCTGATTGCAATGCCAGAACACGTTCGGGATACGTTTGG
GTTTGCTCTTTGGCTTGCGCAACAGGGTAAACAGCATTCACAGACTAAACCATTAAAAGGATTTGGTGGAGCGGGGGTTC
TGGAAGTCGTTGAGGATTATCACGGCAACGCCTGGCGGGCGGTTTACACCATCCAGTTGAAAAATGCGGTTTATGTTCTC
CATGTTTTTCAGAAGAAGTCCGTTTCCGGGAAAGCAACCCCGAAACCTGAAATTGACCTGATTTATCAGCGGCTCAAGGC
TGCACAGCGCCATGCGCAGGAGAGTGGATATGTCACATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10917.44 Da
• Isoelectric Point: 4.4488

>AT215464 WP_015702300.1 NZ_CP082856:1772566-1772859 [Haemophilus influenzae]
MTEQLKDFDVAEHLISEEEIQLYLNEILQEDNIELILSSLGDIARARNMSQIARDAGISREGLYKALSGTGNPTFATVMK
VMKALNLQFQVQQSRFA

Nucleotide

• Length: 294 bp

>AT215464 NZ_CP110775:3613643-3613960 [Citrobacter freundii]
ATGTCACATGATGTCGAAACCAGTAGCGGTAATGTTTACGCCGATTTAGGTGCGTATGACGCAGAACAGATGCAGTTGAA
AGCGCAGCTGGCAATGGTGATTGGTGAGATTTTGCAACGCAAAGGGTTAACGCAGCAGCAGGCCGCGCAGTTACTGGGAA
TGACACAGCCTAAACTTTCCAATCTGCTACGCGGCCAGTTTCGCGGTATCAGTGAAGCAAAGATGCTCGAATGCCTGGTG
AGGCTGGGCCGTGATGTAGAGATTGTTGTCGGGAAAGAGGGCAATATGTCAGGAAAGCTGAAAGTGAAATTTGGCTGA

Structures

Toxin

• AlphaFold DB: A0A852PKG5