Detailed information of TA system TA215457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK(toxin)
• Location: 118390..118933
• Replicon: plasmid unnamed3
• Accession: NZ_CP082855
• Organism: Legionella anisa strain FDAARGOS_1480

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A7G4RL43
• Locus tag: K8O89_RS19990
• Protein ID: WP_014845117.1
• Coordinates: 118613..118933 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: K8O89_RS19985
• Protein ID: WP_223499713.1
• Coordinates: 118390..118626 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8O89_RS19960 (K8O89_19870)	113522..116020	+	2499	WP_014845123.1	helicase C-terminal domain-containing protein	-
K8O89_RS19965 (K8O89_19875)	116200..116481	-	282	WP_014845122.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
K8O89_RS19970 (K8O89_19880)	116474..116734	-	261	WP_014845121.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
K8O89_RS19975 (K8O89_19885)	116947..117831	+	885	WP_014845120.1	recombinase family protein	-
K8O89_RS19980 (K8O89_19890)	117958..118284	+	327	WP_014845119.1	hypothetical protein	-
K8O89_RS19985 (K8O89_19895)	118390..118626	+	237	WP_223499713.1	hypothetical protein	Antitoxin
K8O89_RS19990 (K8O89_19900)	118613..118933	+	321	WP_014845117.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K8O89_RS19995 (K8O89_19905)	119371..119859	-	489	WP_014845116.1	class I SAM-dependent methyltransferase	-
K8O89_RS20000 (K8O89_19910)	120129..120662	-	534	WP_061637559.1	IS91 family transposase	-
K8O89_RS20010 (K8O89_19920)	121247..121402	+	156	WP_154080667.1	hypothetical protein	-
K8O89_RS20015 (K8O89_19925)	121767..122354	+	588	WP_014845114.1	Hsp20/alpha crystallin family protein	-
K8O89_RS20020 (K8O89_19930)	122502..122993	+	492	WP_014845113.1	Hsp20/alpha crystallin family protein	-
K8O89_RS20025 (K8O89_19935)	123619..123921	+	303	WP_014845112.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-29	-	1..143699	143699

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-104)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11444.32 Da
• Isoelectric Point: 9.4321

>T215457 WP_014845117.1 NZ_CP082855:118613-118933 [Legionella anisa]
MNRGDVYWVNLDPTTGSEINKLRPCVLVGATPINQARRTVVVVPLSSSATARPPITITVSCLGKQVTAVCDQIRTIDKSR
LKKVAGNLSDKDLNALDDGLRQVLCL

Nucleotide

• Length: 321 bp

>T215457 NZ_CP110774:3354286-3354804 [Acidocella sp. MX-AZ03]
GTGAGACGTCGGTTGTTGCCGCCGGAACGCCTGACCGCAGCACACGATGTCTCAGGCTTCCAGAATGGCCGCCACCCGTC
CTTGGACGAATGGCTGCGGGACCGGGCATTGGTCAGTGAGGGGCTATCAGCGAGAACCTATGTTGTACGCGCTGGCGAGC
CGCCACATCTCGTCGTCGGCTATTATGCGATCACCACGGCCATGGAGCAGCGCTTGTCCCTGCCCAGCGCCAAGCTGCGG
CGCGGAATGCCCGAGCAGATACCGCTGATGCTAATCGGCCGCTTGGCGGTGGACCAGCGTTACCATGGCATTGGCCTGGG
CACCGACTTATTGGCGGATGCATTGAAGCGTTGCCTCGCCGTGTCCGAGGTGGCGGGCGTCAGGGCGGTGGTCACCCACG
CGATCGATGAGACCGCCATGGCGTTCTATCGGCGGCACGGCTTTTTGGCCTCGCCGTTGGGCGAGAAGGTGCTGATGATG
CCGATTGAGACCATCCGCGCCGTGTTCGCCGATCAGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9063.33 Da
• Isoelectric Point: 4.8047

>AT215457 WP_223499713.1 NZ_CP082855:118390-118626 [Legionella anisa]
MMNKVLISIPDQIASRMRAAIPQRQRSKVIAHLIEKEIERREKALYECALAVENDHGLQNEMNDWDITVQDGLTDESW

Nucleotide

• Length: 237 bp

>AT215457 NZ_CP110774:3354077-3354244 [Acidocella sp. MX-AZ03]
ATGCTGGACAGCGCCCGCCGCCAGGCCGAGGACACGATCCTGGATCAGCGTGTGTTCTTCCTGGCGCCCGATGCGCATGA
GGCGTTTCTGGCGATGCTGGATGCGCCGGCACAGGACGTGGCGGAACTGCGGGCGCGGCTGACCAAGCCGCCAGTCTGGG
ACCGTTAA

Structures

Toxin

• AlphaFold DB: A0A7G4RL43