Detailed information of TA system TA215452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 62805..63348
• Replicon: plasmid unnamed1
• Accession: NZ_CP082853
• Organism: Legionella anisa strain FDAARGOS_1480

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K8O89_RS18605
• Protein ID: WP_058388576.1
• Coordinates: 63058..63348 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: K8O89_RS18600
• Protein ID: WP_058388577.1
• Coordinates: 62805..63065 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8O89_RS18580 (K8O89_18540)	59135..59386	-	252	WP_058388579.1	hypothetical protein	-
K8O89_RS18585 (K8O89_18545)	59697..61019	-	1323	WP_058388578.1	diguanylate cyclase	-
K8O89_RS18590 (K8O89_18550)	61635..61844	+	210	WP_058388586.1	cold-shock protein	-
K8O89_RS18595 (K8O89_18555)	62241..62414	-	174	WP_167344754.1	hypothetical protein	-
K8O89_RS18600 (K8O89_18560)	62805..63065	+	261	WP_058388577.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
K8O89_RS18605 (K8O89_18565)	63058..63348	+	291	WP_058388576.1	type II toxin-antitoxin system YafQ family toxin	Toxin
K8O89_RS18610 (K8O89_18570)	63589..64620	+	1032	WP_058388575.1	SEL1-like repeat protein	-
K8O89_RS18615 (K8O89_18575)	64617..65084	-	468	WP_058388574.1	DUF4156 domain-containing protein	-
K8O89_RS18620 (K8O89_18580)	65348..65521	-	174	WP_167344753.1	hypothetical protein	-
K8O89_RS18625	65756..65905	+	150	WP_202972316.1	hypothetical protein	-
K8O89_RS18630	65911..66129	+	219	WP_238585186.1	carboxymuconolactone decarboxylase family protein	-
K8O89_RS18635 (K8O89_18590)	66110..66424	+	315	WP_161496361.1	FAD-binding oxidoreductase	-
K8O89_RS18640 (K8O89_18595)	66458..67153	+	696	WP_058388572.1	DUF3330 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	lpg2372	1..138031	138031

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (9-96)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-77)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11277.04 Da
• Isoelectric Point: 10.0716

>T215452 WP_058388576.1 NZ_CP082853:63058-63348 [Legionella anisa]
MVNVLKLCSSTQFKKDLKRVAKQGKNRKLIDDVIEKLQRQEPLPQKNRDHALVGDYKGYRECHITPDWLLIYRITNNNAV
ELLELTRTGSHSELFR

Nucleotide

• Length: 291 bp

>T215452 NZ_CP110774:c38026-37595 [Acidocella sp. MX-AZ03]
TTGTATCTCGTCGACACCAATGTCATCTCGGCGCGGGCGCCCTCGCGCGTCCCTCTGCCCGATTTGGCCGCCTGGATGGA
TGCCCACTCGGCCGATTTATTCCTCTCCACGGTCACAATCGCTGAGATCGAGGACGGCATCGCCAAGGCCAAGCGTGAGG
GAGCAACCCGCAAAGCCCGTGACCTTACCGCCTGGCTGGAGACCGTGCTGCACCTTTATGCGGCCCGCATCCTGCCCTTC
GACGTGGCCAGCGCCCGCCTCGCCGGCAGTCTATCAGACTGCGCCCGTGGTCAGGGTCATGCCCCGGGCTTCGCCGACAT
CATCATCGCCGCAACCGCCCAGCACCACGGGCTGACCATCCTGACCCGGAACCTGCGGCATTTTGAGCCGTTGGGCGTGG
CGGTACATGATCCATTTATCCGCCTGCCCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9629.99 Da
• Isoelectric Point: 6.4799

>AT215452 WP_058388577.1 NZ_CP082853:62805-63065 [Legionella anisa]
MGRAASIHARIDPTLKHDAEKILHQLGLNTSQAINAFYAQIVHVRGIPFELKLPNEETIAAMKELDSGKVKTYDSMKDLW
NEIDNG

Nucleotide

• Length: 261 bp

>AT215452 NZ_CP110774:c38272-38030 [Acidocella sp. MX-AZ03]
ATGCGGGAAATCCAACTTCGCGACGCCAAGGCCAGCCTATCGGCTGTGGTCGATGAGGCCCTACGCGGCGAACCCGCGAT
CATCACCCGCCACGGCAAGCCCGAGGCGGTCATTCTCAGCTTCGAGGAATGGCAGCGGCTCTCGCATGTGCCCTCTTTCG
GCCAATTGCTGATGGCGGCACCTCTCGATGCGGACGATCTGCCGCCGCGCAATCCATCTCCGCTCCGTGATAGCGGCCTC
TGA