Detailed information of TA system TA215448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 543430..544967
• Replicon: chromosome
• Accession: NZ_CP082848
• Organism: Legionella pneumophila strain FDAARGOS_1482

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: K8O97_RS02455
• Protein ID: WP_032829405.1
• Coordinates: 543430..544734 (-)
• Length: 435 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: K8O97_RS02460
• Protein ID: WP_014840730.1
• Coordinates: 544728..544967 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8O97_RS02420 (K8O97_02420)	538915..539634	+	720	WP_014843028.1	C39 family peptidase	-
K8O97_RS02425 (K8O97_02425)	539703..540230	+	528	Protein_476	hypothetical protein	-
K8O97_RS02430 (K8O97_02430)	540338..540499	+	162	WP_015444967.1	hypothetical protein	-
K8O97_RS02435 (K8O97_02435)	540536..540940	-	405	WP_011212703.1	MerR family transcriptional regulator	-
K8O97_RS02440 (K8O97_02440)	541010..541762	+	753	WP_027224671.1	glutaredoxin family protein	-
K8O97_RS02445 (K8O97_02445)	541952..543043	+	1092	WP_027224672.1	hypothetical protein	-
K8O97_RS02450 (K8O97_02450)	543085..543360	+	276	Protein_481	integrase	-
K8O97_RS02455 (K8O97_02455)	543430..544734	-	1305	WP_032829405.1	type II toxin-antitoxin system HipA family toxin	Toxin
K8O97_RS02460 (K8O97_02460)	544728..544967	-	240	WP_014840730.1	helix-turn-helix domain-containing protein	Antitoxin
K8O97_RS02465 (K8O97_02465)	545426..545698	+	273	WP_223381015.1	hypothetical protein	-
K8O97_RS02470 (K8O97_02470)	545808..546080	+	273	WP_032829407.1	hypothetical protein	-
K8O97_RS02475 (K8O97_02475)	546775..548187	-	1413	WP_027224675.1	hypothetical protein	-
K8O97_RS02480 (K8O97_02480)	548218..548826	-	609	WP_027224676.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 435 a.a.
• Molecular weight: 49704.13 Da
• Isoelectric Point: 8.2954

>T215448 WP_032829405.1 NZ_CP082848:c544734-543430 [Legionella pneumophila]
MVMRKLYILMNNELVGELTQDNAGGLSFSYVADWISKPHSRPVSLSLPLVNQKFSGEVVYNFFDNLLPDNPQIRARIQAR
FQAKSSRPFDLLALIGSDCVGAIQIVERIPQTQRLSIESHVVSEAEIARTLRSYQTSPLGMDKEDDEFRISIAGAQEKTA
FLYYQNEWHKPFGPTPTTHIFKLPIGYLPHQGIDLSESCENEHLCLLLASKFGLDVAKSKILQFEDEKVLVVERFDRKWS
RDNRYILRLPQEDLCQALGYSPNLKYQSDGGPGIKEIMQLLNGSQQPQKDRDEFFRAQVVYWCLGAIDAHAKNFSISIQP
QGGYQLTPLYDIISAYPIVEQKQLSFKKLKMAMALYGKNIHYKIDGIQKRHFIETAKYVKYSKIKAAQIFEQVIDRIDSA
IDEVSRQLPNHFPEKIADPIFKGMRAFKERMIST

Nucleotide

• Length: 1305 bp

>T215448 NZ_CP110679:2806439-2806708 [Sphingomonas sp. S6-11]
TTGTACATAGAATATATCGAAATTCTCAGCACTTCCGAAGCCCGGGCGAACATCAAGGCGGTGATGGACCGCGTCGTTGC
CAACCGCGCGCCGATCGGCATCTCGCGTCCAAAGGGCGAGGGGGTGGTGATGGTCTCGCAGAGCGAGTGGGATTCGATCG
AGGAGACGATGTATCTGCTGGCGTCGCCCAAGAATGCTGAGCGCTTGCTGGAAGCGATCCGGGGGCTGCAGGCTGGCGGA
GGCGAAGAGCACGAACTGATCGATCCGTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9096.40 Da
• Isoelectric Point: 5.0774

>AT215448 WP_014840730.1 NZ_CP082848:c544967-544728 [Legionella pneumophila]
MIISSAKDLALYLNDKRKQLKMSQSEVADLVGLKQDTISKFENSPDNSRIDTLFRILSALNLNMSLVEKDQKTHEEIEW

Nucleotide

• Length: 240 bp

>AT215448 NZ_CP110679:2806705-2806971 [Sphingomonas sp. S6-11]
GTGAAGATCGTCTTCTGGCCAACCGCCTTGGAGGATTATCTCCATTGGCAGGCCGAAGACACGCGGTTGCTGGAGCGCGT
GAACGCGCTACTCAAGGAGTGCCGGCGCGATCCTTTTCGTGGGCTGGGCAAGCCGGAGCCCCTGGGCAAAAACATGTCCT
GCTGGTGGTCCCGCCGGATCGATCGCGAGCACCGGCTGGTGTATCGCGTGACGGGGAGCGGCGAGGCACAGACCGTGGAA
GTGGCGCAGTGCCGGTATCATTATTGA