Detailed information of TA system TA215444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 4908246..4908889
• Replicon: chromosome
• Accession: NZ_CP082846
• Organism: Mycolicibacterium smegmatis strain FDAARGOS_1484

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K8P01_RS23435
• Protein ID: WP_058125487.1
• Coordinates: 4908500..4908889 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0QRY5
• Locus tag: K8P01_RS23430
• Protein ID: WP_003892665.1
• Coordinates: 4908246..4908500 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8P01_RS23420 (K8P01_23350)	4903676..4907059	+	3384	WP_058125485.1	ATP-binding protein	-
K8P01_RS23425 (K8P01_23355)	4907056..4908225	+	1170	WP_058125486.1	DUF3322 and DUF2220 domain-containing protein	-
K8P01_RS23430 (K8P01_23360)	4908246..4908500	+	255	WP_003892665.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K8P01_RS23435 (K8P01_23365)	4908500..4908889	+	390	WP_058125487.1	type II toxin-antitoxin system VapC family toxin	Toxin
K8P01_RS23440 (K8P01_23370)	4908908..4911313	-	2406	WP_058125488.1	SEC-C metal-binding domain-containing protein	-
K8P01_RS23445 (K8P01_23375)	4911335..4913095	-	1761	WP_058125489.1	sulfatase-like hydrolase/transferase	-
K8P01_RS23450 (K8P01_23380)	4913092..4913529	-	438	WP_058125490.1	SRPBCC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 13894.67 Da
• Isoelectric Point: 4.4731

>T215444 WP_058125487.1 NZ_CP082846:4908500-4908889 [Mycolicibacterium smegmatis]
MVIDTSALVAILTDEPDAELLEGAVADDPVRTMSTASYLETAIVIESRFGEPGGRELDLWLHRASVALVAVDADQADAAR
LAYRRYGKGRHPAGLNYGDCFSYALAKVSGQPLLFKGEDFRLTDVAAVH

Nucleotide

• Length: 390 bp

>T215444 NZ_CP110678:2168158-2168424 [Sphingomonas sp. S1-29]
TTGGCCTGGCGGATTGAGTTCCTGCCCGAGGCGGCGAAGGAACTGGCCAAGCTCGACCGCACCGCAGCGGCGCGGATCAT
CAAGACGTTGGAGACGCGGATCGCCGTGCAGGACGATCCACGTTCGCTGGGAAGCGCGCTGACGGGCGACCATGCGGGCT
ATTGGCGCTGGCGGATCGGCGACTATCGCGTGGTGGCGCGCATCGAGGATGACCGGGTGCTGATCCTGGTAGTGCGAGTG
GCGCATCGGCGCGAGGTGTATCGGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9279.72 Da
• Isoelectric Point: 8.0238

>AT215444 WP_003892665.1 NZ_CP082846:4908246-4908500 [Mycolicibacterium smegmatis]
MALSIKHPEADRLARELAARTGETLTEAVVMALRERLARTVGRTQVVPLREELAAIRRRCAALPVLDDRTAESILGYDDR
GLPS

Nucleotide

• Length: 255 bp

>AT215444 NZ_CP110678:2168497-2168769 [Sphingomonas sp. S1-29]
ATGGCGTACATTGTCGACATGGAAAGCCTGAGCATCTCCGAAGCGCGCGCCAATATGAAGGCGGTGGTCGATCGCGTCGT
GGCCGACAAGGCACCGGTGGCGATCACCCGCCAGCGCGGCGAGGGCGTGGTGATGATCTCGGCGAGCGAATGGGCGTCGA
TCGAGGAAACATTGTACCTGCTGCAGTCGCCGGCGAACGCGCGCGCGCTGATCGAATCGATCGCCGAGCTGGACGCCGGC
AAGGGTAGCGAGCGCGACCTGATCGCGCCGTGA

Structures

Antitoxin

• AlphaFold DB: A0QRY5