Detailed information of TA system TA215441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 2216630..2217271
• Replicon: chromosome
• Accession: NZ_CP082846
• Organism: Mycolicibacterium smegmatis strain FDAARGOS_1484

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: K8P01_RS10560
• Protein ID: WP_011730672.1
• Coordinates: 2216630..2217073 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: A0A2U9PXD6
• Locus tag: K8P01_RS10565
• Protein ID: WP_003897042.1
• Coordinates: 2217083..2217271 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8P01_RS10540 (K8P01_10525)	2212111..2212848	+	738	WP_011730668.1	GntR family transcriptional regulator	-
K8P01_RS10545 (K8P01_10530)	2212850..2214382	+	1533	WP_058126982.1	cyclohexanecarboxylate-CoA ligase	-
K8P01_RS10550 (K8P01_10535)	2214383..2215156	+	774	WP_011730670.1	SDR family oxidoreductase	-
K8P01_RS10555 (K8P01_10540)	2215153..2216625	+	1473	WP_058127741.1	long-chain fatty acid--CoA ligase	-
K8P01_RS10560 (K8P01_10545)	2216630..2217073	-	444	WP_011730672.1	SRPBCC family protein	Toxin
K8P01_RS10565 (K8P01_10550)	2217083..2217271	-	189	WP_003897042.1	antitoxin	Antitoxin
K8P01_RS10570 (K8P01_10555)	2217297..2219672	-	2376	WP_058126983.1	cation-translocating P-type ATPase	-
K8P01_RS10575 (K8P01_10560)	2219686..2221293	-	1608	WP_162267879.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-141)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16157.75 Da
• Isoelectric Point: 9.9432

>T215441 WP_011730672.1 NZ_CP082846:c2217073-2216630 [Mycolicibacterium smegmatis]
MAKLSVSVEVPLPPEQAWEYASDLSRYHEWLSIHRAWRSKLPETLEKGTVIESIVEVKGMLNRVRWTLVHYKPPQALTLN
GDGRGGVKVKLIGKIKPTDNGATVGFDLHLGGPALFGPIGMVVAAALKSDIQESLNRFKQLYAPSAT

Nucleotide

• Length: 444 bp

>T215441 NZ_CP110674:c3802384-3801992 [Mycobacterium tuberculosis]
ATGGCGGCCATCTATCTCGACTCGTCCGCGATCGTCAAGCTCGCGGTTCGCGAGCCAGAGTCGGACGCGCTGCGCCGCTA
CCTTCGCACCCGCCACCCGCGAGTGTCGAGTGCCCTTGCGCGCGCGGAGGTCATGCGGGCCCTGCTTGACAAGGGTGAAT
CAGCGCGGAAAGCCGGCCGCCGGGCGCTGGCTCATCTCGATCTGCTGCGCGTCGACAAGCGCGTCCTCGATCTCGCTGGT
GGGCTGCTGCCATTCGAGCTGCGCACGCTCGATGCCATCCACTTGGCGACGGCGCAGCGGCTCGGCGTCGATCTGGGCCG
GTTGTGCACCTACGACGACCGGATGCGCGACGCCGCCAAGACGCTCGGCATGGCCGTGATCGCCCCGTCGTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6886.64 Da
• Isoelectric Point: 4.6906

>AT215441 WP_003897042.1 NZ_CP082846:c2217271-2217083 [Mycolicibacterium smegmatis]
MGFLDKAKDLLSQNADKVDTVIDKAGDMIDEKTQGKYAQHVDKAQDAAKNAIRKDDPQEPQQ

Nucleotide

• Length: 189 bp

>AT215441 NZ_CP110674:c3802659-3802384 [Mycobacterium tuberculosis]
ATGACCTCGGTGGGGGTGCGTGCGCTGCGGCAGCGGGCCAGCGAGCTGCTGCGCCGAGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGCGGCCGGCCCGTCGCCCTGCTCTCGCCGCTGCCGCAGGGCGGTCCATACGAGCAGCTGCTGGCAAGCG
GCGAGATCGAGCGTGCCACGCTCGACGTCGTTGATCTGCCCGAACCGCTCGACCTCGACGCGGGCGTCGAGCTGCCGTCG
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2U9PXD6