215440

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 1014324..1014865 Replicon chromosome
Accession NZ_CP082846
Organism Mycolicibacterium smegmatis strain FDAARGOS_1484

Toxin (Protein)


Gene name mazF Uniprot ID A0A2U9PVI3
Locus tag K8P01_RS04850 Protein ID WP_003895797.1
Coordinates 1014536..1014865 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0R0N3
Locus tag K8P01_RS04845 Protein ID WP_011729843.1
Coordinates 1014324..1014533 (+) Length 70 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8P01_RS04820 (K8P01_04810) 1009595..1010365 - 771 WP_011729837.1 SDR family oxidoreductase -
K8P01_RS04825 1010902..1011303 - 402 Protein_957 cupin domain-containing protein -
K8P01_RS04830 (K8P01_04820) 1011333..1012253 - 921 WP_011729840.1 NADP-dependent oxidoreductase -
K8P01_RS04835 (K8P01_04825) 1012250..1012534 - 285 WP_011729841.1 hypothetical protein -
K8P01_RS04840 (K8P01_04830) 1012796..1014163 + 1368 WP_081319552.1 dihydrolipoyl dehydrogenase -
K8P01_RS04845 (K8P01_04835) 1014324..1014533 + 210 WP_011729843.1 antitoxin MazE family protein Antitoxin
K8P01_RS04850 (K8P01_04840) 1014536..1014865 + 330 WP_003895797.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
K8P01_RS04855 (K8P01_04845) 1014908..1015471 - 564 WP_014878038.1 helix-turn-helix domain-containing protein -
K8P01_RS04860 (K8P01_04850) 1015577..1016512 + 936 WP_003895799.1 alpha/beta fold hydrolase -
K8P01_RS04865 (K8P01_04855) 1016509..1018221 + 1713 WP_058126565.1 NAD(P)/FAD-dependent oxidoreductase -
K8P01_RS04875 (K8P01_04865) 1018438..1018818 + 381 WP_174519608.1 phage holin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-104)

Antitoxin

Antitoxin MazE-like

(5-68)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 11739.52 Da        Isoelectric Point: 10.4023

>T215440 WP_003895797.1 NZ_CP082846:1014536-1014865 [Mycolicibacterium smegmatis]
VRRGDIYTAAARGAYTGKPRPVLIIQDDRFDATASVTVVPFTTSDTDAPLLRIRIEPTPATGLSTVSSLMIDKVTTVPRS
SLTHRVGRLAETDMVRTDRALLVFLGIAG

Download         Length: 330 bp

>T215440 NZ_CP110674:3773783-3774040 [Mycobacterium tuberculosis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTGGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin


Download         Length: 70 a.a.        Molecular weight: 8114.98 Da        Isoelectric Point: 6.2231

>AT215440 WP_011729843.1 NZ_CP082846:1014324-1014533 [Mycolicibacterium smegmatis]
MTPARDRVRRHRERLRRQGLRPVQIWVPDVNAPEFRREAHRQSELVAAGEHEAEDQAFVDAISVDWDDA

Download         Length: 210 bp

>AT215440 NZ_CP110674:3773511-3773786 [Mycobacterium tuberculosis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U9PVI3

Antitoxin

Source ID Structure
AlphaFold DB A0R0N3

References