Detailed information of TA system TA215439

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 99776..100772
• Replicon: chromosome
• Accession: NZ_CP082846
• Organism: Mycolicibacterium smegmatis strain FDAARGOS_1484

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: K8P01_RS00535
• Protein ID: WP_058126279.1
• Coordinates: 100194..100772 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: I7GB97
• Locus tag: K8P01_RS00530
• Protein ID: WP_003894879.1
• Coordinates: 99776..100168 (+)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8P01_RS00510 (K8P01_00510)	95246..96364	+	1119	WP_003894875.1	acyltransferase	-
K8P01_RS00515 (K8P01_00515)	96538..97143	+	606	WP_003894876.1	class I SAM-dependent methyltransferase	-
K8P01_RS00520 (K8P01_00520)	97145..98773	-	1629	WP_058126278.1	GMC family oxidoreductase N-terminal domain-containing protein	-
K8P01_RS00525 (K8P01_00525)	99083..99712	+	630	WP_003894878.1	FKBP-type peptidyl-prolyl cis-trans isomerase	-
K8P01_RS00530 (K8P01_00530)	99776..100168	+	393	WP_003894879.1	ParD-like family protein	Antitoxin
K8P01_RS00535 (K8P01_00535)	100194..100772	+	579	WP_058126279.1	zeta toxin family protein	Toxin
K8P01_RS00540 (K8P01_00540)	100784..102136	-	1353	WP_011729067.1	alpha/beta hydrolase-fold protein	-
K8P01_RS00545 (K8P01_00545)	102471..102626	+	156	WP_003894882.1	hypothetical protein	-
K8P01_RS00550 (K8P01_00550)	102832..104220	+	1389	WP_058126280.1	L-serine ammonia-lyase	-
K8P01_RS00555 (K8P01_00555)	104254..104814	-	561	WP_058126281.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-136)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21118.31 Da
• Isoelectric Point: 9.9425

>T215439 WP_058126279.1 NZ_CP082846:100194-100772 [Mycolicibacterium smegmatis]
VKRLDLVVGPNGAGKSTFVELTLAPLLTGSAFVNADEIAKRRWPHAPAEHSYDAARIASDTRLKLIELGESFIAETVFSH
PSKLQLIDTAQAAGYTVVMHVVMIPEDLAVLRVQHRVRAGGHHVPEEKIRERHQRLWPLVALAAARADISTFYDNSAVRG
PRIVAQLAGGFAVGRPAWPMWAPQVLTSRWPE

Nucleotide

• Length: 579 bp

>T215439 NZ_CP110674:c3703377-3702976 [Mycobacterium tuberculosis]
GTGACGGTGCTGCTCGACGCCAACGTGCTGATCGCATTGGTGGTCGCCGAGCATGTGCATCATGATGCCGCAGCGGACTG
GCTCATGGCGTCCGACACCGGATTTGCGACCTGCCCGATGACACAAGGAAGCCTGGTTCGATTCCTGGTGCGCTCGGGAC
AGTCCGCGGCGGCGGCTCGGGATGTCGTCAGTGCGGTCCAGTGCACGAGCCGCCACGAATTCTGGCCCGATGCACTCTCT
TTCGCCGGTGTCGAGGTCGCTGGTGTGGTTGGGCACCGGCAGGTGACCGATGCCTACCTTGCCCAGCTCGCGCGAAGCCA
CGACGGGCAGTTGGCGACGCTCGACAGCGGCTTAGCACACCTGCACGGCGACGTCGCGGTACTCATTCCAACGACCACCT
GA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 13841.29 Da
• Isoelectric Point: 4.9246

>AT215439 WP_003894879.1 NZ_CP082846:99776-100168 [Mycolicibacterium smegmatis]
MTETADRVTRFAADLVESAAAEGARQSRSAKQQLDHWARVGRAVSSQHTAARRRVEAALAGDLALRDLTPEEGVVFNAEI
SAAIEENLAHTDYGQVLAGRGVTTVALDDDGAIVRYQPDGTTTVLAPAQR

Nucleotide

• Length: 393 bp

>AT215439 NZ_CP110674:c3703601-3703374 [Mycobacterium tuberculosis]
GTGCGCACTACCATCGACCTGCCGCAAGACCTGCACAAGCAGGCACTGGCGATTGCCCGGGATACGCACCGCACGTTGAG
TGAAACGGTCGCCGACCTCATGCGACGAGGCCTGGCCGCCAACCGCCCTACCGCGTTGTCCTCAGACCCCAGAACGGGAT
TGCCTTTGGTGAGCGTCGGGACCGTCGTGACCTCCGAGGACGTGCGTTCATTAGAGGACGAGCAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2U9PRP8