Detailed information of TA system TA215437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-YefM
• Location: 2322418..2322920
• Replicon: chromosome
• Accession: NZ_CP082844
• Organism: Nocardia asteroides strain FDAARGOS_1485

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K8O92_RS10545
• Protein ID: WP_195072031.1
• Coordinates: 2322663..2322920 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: K8O92_RS10540
• Protein ID: WP_159927894.1
• Coordinates: 2322418..2322666 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8O92_RS10510 (K8O92_10475)	2317498..2318091	-	594	WP_159927873.1	TetR family transcriptional regulator	-
K8O92_RS10515 (K8O92_10480)	2318151..2318726	+	576	WP_223520118.1	dihydrofolate reductase family protein	-
K8O92_RS10520 (K8O92_10485)	2318758..2319333	+	576	WP_223520120.1	dihydrofolate reductase family protein	-
K8O92_RS10525 (K8O92_10490)	2319389..2319802	-	414	WP_159927879.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
K8O92_RS10530 (K8O92_10495)	2319967..2321232	+	1266	WP_159927881.1	aspartate kinase	-
K8O92_RS10535 (K8O92_10500)	2321236..2322270	+	1035	WP_159927892.1	aspartate-semialdehyde dehydrogenase	-
K8O92_RS10540 (K8O92_10505)	2322418..2322666	+	249	WP_159927894.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
K8O92_RS10545 (K8O92_10510)	2322663..2322920	+	258	WP_195072031.1	Txe/YoeB family addiction module toxin	Toxin
K8O92_RS10550 (K8O92_10515)	2322985..2323908	+	924	WP_223520122.1	aminotransferase class IV	-
K8O92_RS10555 (K8O92_10520)	2323925..2325274	-	1350	WP_223520124.1	serine hydrolase	-
K8O92_RS10560 (K8O92_10525)	2325404..2326663	-	1260	WP_223520127.1	APC family permease	-
K8O92_RS10565 (K8O92_10530)	2326784..2327242	+	459	WP_223520129.1	hypothetical protein	-
K8O92_RS10570 (K8O92_10535)	2327349..2327873	-	525	WP_223520131.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (4-69)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10025.60 Da
• Isoelectric Point: 9.4195

>T215437 WP_195072031.1 NZ_CP082844:2322663-2322920 [Nocardia asteroides]
MKVVFSESAWEEYSAWLQRDRLVLKKVNVLIKAILSDPFEGIGKPEPLKHDLSGYWSRRITQEHRIVYRVVDDAIWIAKV
GKHYD

Nucleotide

• Length: 258 bp

>T215437 NZ_CP110674:c3696101-3695694 [Mycobacterium tuberculosis]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8911.12 Da
• Isoelectric Point: 4.8096

>AT215437 WP_159927894.1 NZ_CP082844:2322418-2322666 [Nocardia asteroides]
MAAAITASEARKNLFPLIRKVNEDHDTIEIVSKGGNAVLISKEDYDSLMTTVELLSGRDGARLRRSIQELEGGGGVVRDL
IE

Nucleotide

• Length: 249 bp

>AT215437 NZ_CP110674:c3696358-3696098 [Mycobacterium tuberculosis]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA