Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2450922..2451540 Replicon chromosome
Accession NZ_CP082841
Organism Kluyvera sp. CRP

Toxin (Protein)


Gene name Hha Uniprot ID A0A1B7JI95
Locus tag K7B04_RS11515 Protein ID WP_064547995.1
Coordinates 2450922..2451140 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1B7JI67
Locus tag K7B04_RS11520 Protein ID WP_064547997.1
Coordinates 2451166..2451540 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K7B04_RS11485 (K7B04_11450) 2447036..2447299 + 264 WP_223804049.1 type B 50S ribosomal protein L31 -
K7B04_RS11490 (K7B04_11455) 2447299..2447439 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
K7B04_RS11495 (K7B04_11460) 2447436..2448134 - 699 WP_163445948.1 GNAT family protein -
K7B04_RS11500 (K7B04_11465) 2448238..2449689 + 1452 WP_223804050.1 PLP-dependent aminotransferase family protein -
K7B04_RS11505 (K7B04_11470) 2449658..2450128 - 471 WP_163445950.1 YlaC family protein -
K7B04_RS11510 (K7B04_11475) 2450234..2450788 - 555 WP_223804051.1 maltose O-acetyltransferase -
K7B04_RS11515 (K7B04_11480) 2450922..2451140 - 219 WP_064547995.1 HHA domain-containing protein Toxin
K7B04_RS11520 (K7B04_11485) 2451166..2451540 - 375 WP_064547997.1 Hha toxicity modulator TomB Antitoxin
K7B04_RS11525 (K7B04_11490) 2452076..2455222 - 3147 WP_064547999.1 multidrug efflux RND transporter permease subunit AcrB -
K7B04_RS11530 (K7B04_11495) 2455245..2456441 - 1197 WP_163445952.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-115)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8629.97 Da        Isoelectric Point: 8.8580

>T215427 WP_064547995.1 NZ_CP082841:c2451140-2450922 [Kluyvera sp. CRP]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSTVWKYIR

Download         Length: 219 bp

>T215427 NZ_CP110674:2946293-2946556 [Mycobacterium tuberculosis]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGAATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14369.19 Da        Isoelectric Point: 4.8781

>AT215427 WP_064547997.1 NZ_CP082841:c2451540-2451166 [Kluyvera sp. CRP]
MDEYSPKRHDIAQLKFLCETLYHDCLATLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYDDDNKLIAQIDEYL
DDTFMLFSNYGINSVDLQKWRKSGNKLFRCFVNASRANPVSLSC

Download         Length: 375 bp

>AT215427 NZ_CP110674:2946008-2946289 [Mycobacterium tuberculosis]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JI95

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JI67

References