Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5364362..5364987 Replicon chromosome
Accession NZ_CP082840
Organism Klebsiella pneumoniae strain W09308

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K6041_RS26445 Protein ID WP_002882817.1
Coordinates 5364362..5364745 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K6041_RS26450 Protein ID WP_004150355.1
Coordinates 5364745..5364987 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6041_RS26430 (K6041_26380) 5361728..5362630 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K6041_RS26435 (K6041_26385) 5362627..5363262 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K6041_RS26440 (K6041_26390) 5363259..5364188 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K6041_RS26445 (K6041_26395) 5364362..5364745 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K6041_RS26450 (K6041_26400) 5364745..5364987 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K6041_RS26455 (K6041_26405) 5365192..5366109 + 918 WP_002882812.1 alpha/beta hydrolase -
K6041_RS26460 (K6041_26410) 5366123..5367064 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K6041_RS26465 (K6041_26415) 5367109..5367546 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K6041_RS26470 (K6041_26420) 5367543..5368403 - 861 WP_002882807.1 virulence factor BrkB family protein -
K6041_RS26475 (K6041_26425) 5368397..5368996 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T215419 WP_002882817.1 NZ_CP082840:c5364745-5364362 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T215419 NZ_CP110674:2643337-2643714 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT215419 WP_004150355.1 NZ_CP082840:c5364987-5364745 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT215419 NZ_CP110674:2643083-2643340 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References