Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4184857..4185476 Replicon chromosome
Accession NZ_CP082840
Organism Klebsiella pneumoniae strain W09308

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag K6041_RS20770 Protein ID WP_002892050.1
Coordinates 4185258..4185476 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag K6041_RS20765 Protein ID WP_002892066.1
Coordinates 4184857..4185231 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6041_RS20755 (K6041_20710) 4180009..4181202 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K6041_RS20760 (K6041_20715) 4181225..4184371 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
K6041_RS20765 (K6041_20720) 4184857..4185231 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
K6041_RS20770 (K6041_20725) 4185258..4185476 + 219 WP_002892050.1 HHA domain-containing protein Toxin
K6041_RS20775 (K6041_20730) 4185635..4186201 + 567 WP_002892030.1 maltose O-acetyltransferase -
K6041_RS20780 (K6041_20735) 4186173..4186313 - 141 WP_004147370.1 hypothetical protein -
K6041_RS20785 (K6041_20740) 4186334..4186804 + 471 WP_002892026.1 YlaC family protein -
K6041_RS20790 (K6041_20745) 4186779..4188230 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
K6041_RS20795 (K6041_20750) 4188331..4189029 + 699 WP_002892021.1 GNAT family protein -
K6041_RS20800 (K6041_20755) 4189026..4189166 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
K6041_RS20805 (K6041_20760) 4189166..4189429 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T215415 WP_002892050.1 NZ_CP082840:4185258-4185476 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T215415 NZ_CP110674:c2180317-2180000 [Mycobacterium tuberculosis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT215415 WP_002892066.1 NZ_CP082840:4184857-4185231 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT215415 NZ_CP110674:c2180529-2180314 [Mycobacterium tuberculosis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References