Detailed information of TA system TA021540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 2623770..2624479
• Replicon: chromosome
• Accession: NC_007948
• Organism: Polaromonas sp. JS666

Toxin (Protein)

• Gene name: tad
• Uniprot ID: Q12AL6
• Locus tag: BPRO_RS12465
• Protein ID: WP_011483424.1
• Coordinates: 2623770..2624153 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: BPRO_RS12470
• Protein ID: WP_011483425.1
• Coordinates: 2624150..2624479 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BPRO_RS12435	2619368..2620567	-	1200	WP_011483418.1	chromate transporter	-
BPRO_RS12440	2620859..2621248	-	390	WP_157045793.1	hypothetical protein	-
BPRO_RS12445	2621427..2621777	+	351	WP_011483420.1	hypothetical protein	-
BPRO_RS12450	2622038..2622793	+	756	WP_041389724.1	HupE/UreJ family protein	-
BPRO_RS12455	2622831..2623181	+	351	WP_011483422.1	hypothetical protein	-
BPRO_RS28020	2623323..2623637	+	315	WP_011483423.1	hypothetical protein	-
BPRO_RS12465	2623770..2624153	+	384	WP_011483424.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BPRO_RS12470	2624150..2624479	+	330	WP_011483425.1	helix-turn-helix domain-containing protein	Antitoxin
BPRO_RS12475	2624576..2625892	+	1317	WP_041389728.1	site-specific integrase	-
BPRO_RS12480	2626039..2629008	+	2970	WP_011483427.1	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2624546..2625892	1346

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (18-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-90)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14086.21 Da
• Isoelectric Point: 10.0152

>T21540 WP_011483424.1 NC_007948:2623770-2624153 [Polaromonas sp. JS666]
MLKQARKPIKWVGSTKRDLDAMPEDVKDVFGHAIDLAQAGGKHQDAKVMTGFGSAGVLEVVEDHQGDTFRAVYTVKFAGW
VYVLHCFQKKSKSGIATPKPDMDVINIRLKAAKQDFEAWQAQQGARK

Nucleotide

• Length: 384 bp

>T21540 NC_007948:2623770-2624153 [Polaromonas sp. JS666]
ATGTTGAAGCAAGCGCGCAAACCCATCAAATGGGTCGGTTCCACCAAGCGCGATCTGGATGCGATGCCGGAGGATGTCAA
AGATGTCTTCGGTCATGCCATCGACCTGGCACAAGCCGGCGGCAAGCACCAGGATGCCAAGGTGATGACCGGCTTCGGCT
CGGCCGGCGTGCTGGAGGTGGTCGAGGATCATCAGGGTGACACTTTTCGGGCCGTTTACACCGTCAAGTTCGCCGGGTGG
GTTTATGTCCTGCACTGCTTCCAGAAGAAGTCCAAAAGCGGCATCGCCACGCCGAAGCCGGACATGGACGTGATCAATAT
CCGCCTGAAAGCCGCGAAGCAGGATTTCGAAGCATGGCAGGCACAGCAAGGAGCACGCAAATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11914.63 Da
• Isoelectric Point: 10.0877

>AT21540 WP_011483425.1 NC_007948:2624150-2624479 [Polaromonas sp. JS666]
MNGHNEITIEEGGANVYADLGYTDAAEMQRKSQLAGEIARAIKARRLTQEDAATLLGIDQSKVSRITRGQFRGVSEAKLL
ELVARLGHDVKIVVGPVRRRAGKIELQFA

Nucleotide

• Length: 330 bp

>AT21540 NC_007948:2624150-2624479 [Polaromonas sp. JS666]
ATGAATGGGCACAACGAAATCACGATCGAAGAAGGCGGCGCCAATGTCTATGCCGACCTGGGTTATACCGACGCTGCGGA
AATGCAGCGAAAATCCCAGCTCGCCGGCGAGATCGCCCGCGCGATCAAGGCGCGTCGCCTGACGCAAGAAGATGCGGCGA
CGCTGCTGGGCATTGATCAATCCAAGGTCTCGCGCATCACGCGCGGCCAATTCCGCGGCGTCAGCGAAGCCAAGCTACTG
GAGCTGGTGGCCAGGCTGGGGCACGATGTAAAGATCGTGGTCGGCCCGGTGCGGCGGCGCGCCGGCAAGATCGAGCTGCA
GTTTGCCTGA

Structures

Toxin

• AlphaFold DB: Q12AL6