Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 23674..24233 | Replicon | plasmid unnamed1 |
| Accession | NZ_CP082836 | ||
| Organism | Escherichia coli strain 1613 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | O82928 |
| Locus tag | K8O78_RS27435 | Protein ID | WP_000421248.1 |
| Coordinates | 23674..23949 (-) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | C1J8K5 |
| Locus tag | K8O78_RS27440 | Protein ID | WP_001178089.1 |
| Coordinates | 23949..24233 (-) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| K8O78_RS27400 (19027) | 19027..19416 | - | 390 | WP_001172748.1 | cytochrome b562 | - |
| K8O78_RS27405 (19460) | 19460..21670 | - | 2211 | WP_000592771.1 | catalase/peroxidase KatP | - |
| K8O78_RS27410 (21847) | 21847..22032 | + | 186 | WP_001302179.1 | hypothetical protein | - |
| K8O78_RS27415 (22032) | 22032..22436 | + | 405 | Protein_16 | IS91 family transposase | - |
| K8O78_RS27420 (22448) | 22448..23014 | + | 567 | Protein_17 | IS3-like element IS600 family transposase | - |
| K8O78_RS27425 (23058) | 23058..23273 | + | 216 | Protein_18 | transposase | - |
| K8O78_RS27430 (23373) | 23373..23579 | - | 207 | WP_001105064.1 | hypothetical protein | - |
| K8O78_RS27435 (23674) | 23674..23949 | - | 276 | WP_000421248.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| K8O78_RS27440 (23949) | 23949..24233 | - | 285 | WP_001178089.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| K8O78_RS27445 (25144) | 25144..26001 | - | 858 | WP_000130945.1 | incFII family plasmid replication initiator RepA | - |
| K8O78_RS27450 (25994) | 25994..26068 | - | 75 | WP_001370046.1 | RepA leader peptide Tap | - |
| K8O78_RS27455 (26304) | 26304..26558 | - | 255 | WP_000083831.1 | replication regulatory protein RepA | - |
| K8O78_RS27460 (26798) | 26798..27136 | - | 339 | WP_000766796.1 | hypothetical protein | - |
| K8O78_RS27465 (27174) | 27174..27383 | - | 210 | WP_001302200.1 | hemolysin expression modulator Hha | - |
| K8O78_RS27470 (27429) | 27429..27890 | - | 462 | WP_001233853.1 | thermonuclease family protein | - |
| K8O78_RS27475 (28135) | 28135..28347 | - | 213 | WP_001302189.1 | ANR family transcriptional regulator | - |
| K8O78_RS27480 (28480) | 28480..29040 | - | 561 | WP_000840472.1 | fertility inhibition protein FinO | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | stcE / espP / toxB / hlyD / hlyB / hlyA / hlyC / exeG / exeE | 1..92294 | 92294 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10735.42 Da Isoelectric Point: 9.9324
>T215399 WP_000421248.1 NZ_CP082836:c23949-23674 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T215399 NZ_CP110674:c1168090-1167797 [Mycobacterium tuberculosis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA
Antitoxin
Download Length: 95 a.a. Molecular weight: 10474.89 Da Isoelectric Point: 5.6558
>AT215399 WP_001178089.1 NZ_CP082836:c24233-23949 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
Download Length: 285 bp
>AT215399 NZ_CP110674:c1168356-1168087 [Mycobacterium tuberculosis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z6FAZ0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A731IHA8 |