Detailed information of TA system TA021539

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 468401..468917
• Replicon: chromosome
• Accession: NC_007948
• Organism: Polaromonas sp. JS666

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q12GC6
• Locus tag: BPRO_RS02245
• Protein ID: WP_011481423.1
• Coordinates: 468654..468917 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: Q12GC7
• Locus tag: BPRO_RS02240
• Protein ID: WP_011481422.1
• Coordinates: 468401..468664 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BPRO_RS02215	463815..464810	+	996	WP_011481417.1	fumarylacetoacetate hydrolase family protein	-
BPRO_RS02220	464851..465453	-	603	WP_011481418.1	MarC family protein	-
BPRO_RS02225	465513..465926	-	414	WP_011481419.1	VOC family protein	-
BPRO_RS02230	466124..467017	+	894	WP_041388248.1	hypothetical protein	-
BPRO_RS02235	467046..468338	+	1293	WP_011481421.1	FIST C-terminal domain-containing protein	-
BPRO_RS02240	468401..468664	-	264	WP_011481422.1	hypothetical protein	Antitoxin
BPRO_RS02245	468654..468917	-	264	WP_011481423.1	BrnT family toxin	Toxin
BPRO_RS29260	469092..469679	+	588	Protein_452	IS5/IS1182 family transposase	-
BPRO_RS02250	469686..470417	-	732	WP_011481425.1	helix-turn-helix transcriptional regulator	-
BPRO_RS02255	470797..471603	+	807	WP_011481427.1	hypothetical protein	-
BPRO_RS02260	471684..473645	+	1962	WP_011481428.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	457734..468917	11183

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10160.50 Da
• Isoelectric Point: 6.2233

>T21539 WP_011481423.1 NC_007948:c468917-468654 [Polaromonas sp. JS666]
MNSFEFDESKSESNFLKHHINFIDAQLLWNDPRLLEIPAKTEDEPRYLVIGLINGKHWSAVVTYRGANIRLISVRRARTE
EVALYES

Nucleotide

• Length: 264 bp

>T21539 NC_007948:c468917-468654 [Polaromonas sp. JS666]
ATGAACTCCTTCGAATTTGACGAATCCAAAAGCGAGTCCAACTTTTTAAAGCACCACATCAACTTTATTGATGCGCAGCT
TCTATGGAATGATCCACGGCTATTGGAGATTCCGGCAAAAACGGAGGATGAGCCCCGGTATCTGGTGATCGGGCTTATCA
ATGGCAAACACTGGTCAGCGGTGGTCACCTACAGGGGTGCCAATATTCGACTTATTTCTGTCCGTCGTGCGCGAACCGAG
GAGGTAGCACTGTATGAAAGCTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9955.37 Da
• Isoelectric Point: 10.3815

>AT21539 WP_011481422.1 NC_007948:c468664-468401 [Polaromonas sp. JS666]
MKAKTFEQQFDEGVDITASLDLSKAKRVLQVQKRVNVDFPAWMIDSLDREASKLGVTRQSVIKVWLAERLEESASNTSRR
RMRAEGT

Nucleotide

• Length: 264 bp

>AT21539 NC_007948:c468664-468401 [Polaromonas sp. JS666]
ATGAAAGCTAAAACTTTTGAGCAACAGTTTGACGAGGGCGTGGACATCACGGCTTCTCTGGATTTATCCAAAGCAAAACG
TGTGCTGCAGGTGCAAAAGCGCGTGAATGTCGATTTCCCTGCGTGGATGATTGACTCGCTGGACCGGGAAGCCAGCAAGC
TCGGCGTCACCCGGCAGTCGGTTATCAAGGTCTGGCTTGCTGAGCGACTTGAGGAGTCCGCTTCCAACACCTCTCGCCGA
CGAATGCGCGCCGAGGGGACGTAG

Structures

Toxin

• AlphaFold DB: Q12GC6

Antitoxin

• AlphaFold DB: Q12GC7