Detailed information of TA system TA215388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4292147..4292901
• Replicon: chromosome
• Accession: NZ_CP082835
• Organism: Escherichia coli strain 1613

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: K8O78_RS21705
• Protein ID: WP_001301452.1
• Coordinates: 4292416..4292901 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: Q8X700
• Locus tag: K8O78_RS21700
• Protein ID: WP_000801912.1
• Coordinates: 4292147..4292425 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8O78_RS21695 (4289375)	4289375..4292080	+	2706	WP_000906970.1	HTH-type transcriptional regulator MalT	-
K8O78_RS21700 (4292147)	4292147..4292425	+	279	WP_000801912.1	DUF1778 domain-containing protein	Antitoxin
K8O78_RS21705 (4292416)	4292416..4292901	+	486	WP_001301452.1	GNAT family N-acetyltransferase	Toxin
K8O78_RS21710 (4292951)	4292951..4293979	-	1029	WP_000827117.1	RNA 3'-terminal phosphate cyclase	-
K8O78_RS21715 (4293983)	4293983..4295209	-	1227	WP_001105473.1	RNA-splicing ligase RtcB	-
K8O78_RS21720 (4295397)	4295397..4296995	+	1599	WP_001232889.1	DNA-binding transcriptional regulator RtcR	-
K8O78_RS21725 (4296977)	4296977..4297735	-	759	WP_001302007.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17650.57 Da
• Isoelectric Point: 9.4066

>T215388 WP_001301452.1 NZ_CP082835:4292416-4292901 [Escherichia coli]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Nucleotide

• Length: 486 bp

>T215388 NZ_CP110674:361812-362114 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10420.94 Da
• Isoelectric Point: 4.6336

>AT215388 WP_000801912.1 NZ_CP082835:4292147-4292425 [Escherichia coli]
MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT215388 NZ_CP110674:362162-362383 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LHB9