Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hicAB/- |
| Location | 5787..6379 | Replicon | plasmid pCRTS |
| Accession | NZ_CP082834 | ||
| Organism | Citrobacter rodentium NBRC 105723 = DSM 16636 | ||
Toxin (Protein)
| Gene name | hicA | Uniprot ID | V0AGQ2 |
| Locus tag | K7R23_RS25565 | Protein ID | WP_000520549.1 |
| Coordinates | 5787..6035 (+) | Length | 83 a.a. |
Antitoxin (Protein)
| Gene name | hicB | Uniprot ID | V0AWG0 |
| Locus tag | K7R23_RS25570 | Protein ID | WP_000681613.1 |
| Coordinates | 6032..6379 (+) | Length | 116 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| K7R23_RS25520 (K7R23_25330) | 1129..1380 | + | 252 | WP_000121743.1 | plasmid stabilization protein | - |
| K7R23_RS25525 (K7R23_25335) | 1370..1651 | + | 282 | WP_000220561.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| K7R23_RS25530 (K7R23_25340) | 1675..2024 | + | 350 | Protein_2 | DNA-binding protein | - |
| K7R23_RS25535 (K7R23_25345) | 2662..3177 | + | 516 | WP_001025397.1 | J domain-containing protein | - |
| K7R23_RS25540 (K7R23_25350) | 3282..3824 | + | 543 | WP_012908950.1 | hypothetical protein | - |
| K7R23_RS25545 (K7R23_25355) | 3821..4072 | + | 252 | WP_001230707.1 | hypothetical protein | - |
| K7R23_RS25550 (K7R23_25360) | 4059..4286 | + | 228 | WP_000801440.1 | hypothetical protein | - |
| K7R23_RS25555 (K7R23_25365) | 4740..5039 | + | 300 | WP_000650311.1 | hypothetical protein | - |
| K7R23_RS25560 (K7R23_25370) | 5131..5481 | + | 351 | WP_000854261.1 | hypothetical protein | - |
| K7R23_RS25565 (K7R23_25375) | 5787..6035 | + | 249 | WP_000520549.1 | type II toxin-antitoxin system HicA family toxin | Toxin |
| K7R23_RS25570 (K7R23_25380) | 6032..6379 | + | 348 | WP_000681613.1 | type II toxin-antitoxin system HicB family antitoxin | Antitoxin |
| K7R23_RS25575 (K7R23_25385) | 6743..7246 | + | 504 | WP_000781818.1 | DNA distortion polypeptide 1 | - |
| K7R23_RS25580 (K7R23_25390) | 7250..8479 | + | 1230 | WP_000539545.1 | DNA relaxase/nickase TaxC | - |
| K7R23_RS25585 (K7R23_25395) | 8556..8822 | + | 267 | WP_000160396.1 | hypothetical protein | - |
| K7R23_RS25590 (K7R23_25400) | 8827..9060 | + | 234 | WP_000121162.1 | EexN family lipoprotein | - |
| K7R23_RS25595 (K7R23_25405) | 9498..10118 | + | 621 | WP_012908959.1 | lytic transglycosylase domain-containing protein | - |
| K7R23_RS25600 (K7R23_25410) | 10132..10458 | + | 327 | WP_000008647.1 | TrbC/VirB2 family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..39262 | 39262 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 83 a.a. Molecular weight: 9422.08 Da Isoelectric Point: 10.4800
>T215360 WP_000520549.1 NZ_CP082834:5787-6035 [Citrobacter rodentium NBRC 105723 = DSM 16636]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL
Download Length: 249 bp
>T215360 NZ_CP110661:c335885-335667 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA
Antitoxin
Download Length: 116 a.a. Molecular weight: 13113.84 Da Isoelectric Point: 4.6723
>AT215360 WP_000681613.1 NZ_CP082834:6032-6379 [Citrobacter rodentium NBRC 105723 = DSM 16636]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA
Download Length: 348 bp
>AT215360 NZ_CP110661:c336286-335912 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CIX3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CLC1 |