Detailed information of TA system TA215337

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 401143..401797
• Replicon: chromosome
• Accession: NZ_CP082833
• Organism: Citrobacter rodentium NBRC 105723 = DSM 16636

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: D2TS18
• Locus tag: K7R23_RS01815
• Protein ID: WP_012908780.1
• Coordinates: 401143..401550 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: D2TS17
• Locus tag: K7R23_RS01820
• Protein ID: WP_012908779.1
• Coordinates: 401531..401797 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K7R23_RS01795 (K7R23_01765)	397105..398838	-	1734	WP_012908784.1	single-stranded-DNA-specific exonuclease RecJ	-
K7R23_RS01800 (K7R23_01770)	398844..399557	-	714	WP_012908783.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
K7R23_RS01805 (K7R23_01775)	399581..400477	-	897	WP_012908782.1	site-specific tyrosine recombinase XerD	-
K7R23_RS01810 (K7R23_01780)	400578..401099	+	522	WP_012908781.1	flavodoxin FldB	-
K7R23_RS01815 (K7R23_01785)	401143..401550	-	408	WP_012908780.1	protein YgfX	Toxin
K7R23_RS01820 (K7R23_01790)	401531..401797	-	267	WP_012908779.1	FAD assembly factor SdhE	Antitoxin
K7R23_RS01825 (K7R23_01795)	402054..403034	+	981	WP_012908778.1	tRNA-modifying protein YgfZ	-
K7R23_RS01830 (K7R23_01800)	403116..403778	-	663	WP_012908777.1	hemolysin III family protein	-
K7R23_RS01835 (K7R23_01805)	403941..404252	-	312	WP_012908776.1	N(4)-acetylcytidine aminohydrolase	-
K7R23_RS01840 (K7R23_01810)	404442..405876	+	1435	Protein_362	6-phospho-beta-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-77)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15724.61 Da
• Isoelectric Point: 9.4464

>T215337 WP_012908780.1 NZ_CP082833:c401550-401143 [Citrobacter rodentium NBRC 105723 = DSM 16636]
VVLWQSDLRVSWRAQWLSLLLHGLVAAAILLMPWPLSYTPLWLLLLSLVVFDCVRSQRRINACQGEVKLLMDGRLHWQGQ
DWSVISAPWMMKAGMMLRLRSDGGKRQHLWIAADSMDEAEWRDLCRLMLQPSTQR

Nucleotide

• Length: 408 bp

>T215337 NZ_CP110650:c1504755-1504435 [Aeromonas salmonicida]
ATGAAAGACCATGAGCAGACCGTCACATTCAGTCGTTATGATGCGGCTGACTACCTCAAGACCGATGAAGACATGCTCCA
CTATTTGGAGGCCGTCATGGAAGAGGGCGATCCCGCCTTGATTGCAGCCGCCCTCGGTGACATCGCCCGCGCTCGCAACC
TGAGCCAGCTGGCCCGTGATGTTGGGATGAGCAGAGAGGGGCTATACAAAGCCTTATCCGGAGAAGGCAACCCGACCTTT
GCCACCATAACCAAAGTGGCGGGCGCACTGGGGCTGCGCTTTGGTGTCCACCTTGCCAATCCGCCAGCAACCCACGCGTG
A

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10498.08 Da
• Isoelectric Point: 5.6311

>AT215337 WP_012908779.1 NZ_CP082833:c401797-401531 [Citrobacter rodentium NBRC 105723 = DSM 16636]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDAEKRIFVRLLECDDPDLFNWLMNHGKPADAELEQMVRLIQIRN
RDRGPVAI

Nucleotide

• Length: 267 bp

>AT215337 NZ_CP110650:c1505054-1504758 [Aeromonas salmonicida]
ATGAGAGAGATAATCAAAACAAACGTGTTCGACCGCTGGTTTGACTCCCTGCGAGATGCTCGCGCACAGGCAAAGGTGAC
AGCCAGGATCCGGCGCTTGGGTCTTGGCAATCCTGGGGACGTTAAACCTATCGGGGAGGGTCTATCGGAAATGCGGATAG
ACTACGGCCCCGGGTACAGAGTGTATTTTATGACAAAAGGCCCGATCATCATCGTGCTGCTGTGCGGTGGGGATAAGGGC
ACCCAAGCCCGTGATATCGAACAGGCAAAAACCATTGCCGCGCAATGGAAGGATTAA

Structures

Toxin

• AlphaFold DB: D2TS18

Antitoxin

• AlphaFold DB: D2TS17