Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4427124..4427959 Replicon chromosome
Accession NZ_CP082831
Organism Escherichia coli strain SCAID WND1-2021 (1/128)

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0J2AEA6
Locus tag K8B75_RS21865 Protein ID WP_000854759.1
Coordinates 4427124..4427501 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NM52
Locus tag K8B75_RS21870 Protein ID WP_001295723.1
Coordinates 4427591..4427959 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B75_RS21835 (4423062) 4423062..4423238 - 177 Protein_4318 helix-turn-helix domain-containing protein -
K8B75_RS21840 (4423430) 4423430..4424176 - 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
K8B75_RS21845 (4424191) 4424191..4425732 - 1542 WP_001298859.1 IS21-like element ISEc12 family transposase -
K8B75_RS21850 (4426191) 4426191..4426340 - 150 Protein_4321 hypothetical protein -
K8B75_RS21855 (4426446) 4426446..4426622 - 177 WP_000839286.1 DUF957 domain-containing protein -
K8B75_RS21860 (4426639) 4426639..4427127 - 489 WP_000761690.1 DUF5983 family protein -
K8B75_RS21865 (4427124) 4427124..4427501 - 378 WP_000854759.1 TA system toxin CbtA family protein Toxin
K8B75_RS21870 (4427591) 4427591..4427959 - 369 WP_001295723.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
K8B75_RS21875 (4428122) 4428122..4428343 - 222 WP_000692345.1 DUF987 domain-containing protein -
K8B75_RS21880 (4428406) 4428406..4428882 - 477 WP_001186775.1 RadC family protein -
K8B75_RS21885 (4428898) 4428898..4429371 - 474 WP_001350782.1 antirestriction protein -
K8B75_RS21890 (4429713) 4429713..4430531 - 819 WP_001234738.1 DUF932 domain-containing protein -
K8B75_RS21895 (4430649) 4430649..4430844 - 196 Protein_4330 DUF905 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14144.26 Da        Isoelectric Point: 7.3249

>T215323 WP_000854759.1 NZ_CP082831:c4427501-4427124 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T215323 NZ_CP110638:1994349-1994451 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13592.40 Da        Isoelectric Point: 6.6255

>AT215323 WP_001295723.1 NZ_CP082831:c4427959-4427591 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT215323 NZ_CP110638:c1994458-1994313 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2AEA6

Antitoxin

Source ID Structure
AlphaFold DB S1NM52

References