Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4233746..4234266 Replicon chromosome
Accession NZ_CP082831
Organism Escherichia coli strain SCAID WND1-2021 (1/128)

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag K8B75_RS20940 Protein ID WP_001160964.1
Coordinates 4233746..4234030 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag K8B75_RS20945 Protein ID WP_000125566.1
Coordinates 4234033..4234266 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B75_RS20915 (4229391) 4229391..4230677 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
K8B75_RS20920 (4230677) 4230677..4231666 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
K8B75_RS20925 (4231663) 4231663..4232484 + 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
K8B75_RS20930 (4232487) 4232487..4232864 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
K8B75_RS20935 (4232871) 4232871..4233719 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
K8B75_RS20940 (4233746) 4233746..4234030 - 285 WP_001160964.1 CcdB family protein Toxin
K8B75_RS20945 (4234033) 4234033..4234266 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
K8B75_RS20950 (4234352) 4234352..4234831 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
K8B75_RS20955 (4235023) 4235023..4236885 - 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
K8B75_RS20960 (4236878) 4236878..4237408 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
K8B75_RS20965 (4237516) 4237516..4238847 - 1332 WP_001183209.1 MFS transporter -
K8B75_RS20970 (4238906) 4238906..4239193 - 288 WP_000203744.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T215321 WP_001160964.1 NZ_CP082831:c4234030-4233746 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T215321 NZ_CP110638:353348-353695 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT215321 WP_000125566.1 NZ_CP082831:c4234266-4234033 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT215321 NZ_CP110638:353695-353994 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References