Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2454074..2455662 Replicon chromosome
Accession NZ_CP082831
Organism Escherichia coli strain SCAID WND1-2021 (1/128)

Toxin (Protein)


Gene name hipA Uniprot ID A0A061YM43
Locus tag K8B75_RS11860 Protein ID WP_001125411.1
Coordinates 2454074..2455396 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A061YNW4
Locus tag K8B75_RS11865 Protein ID WP_001296076.1
Coordinates 2455396..2455662 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B75_RS11840 (2450060) 2450060..2450623 - 564 WP_001296074.1 type 1 fimbrial major subunit FimA -
K8B75_RS11845 (2451262) 2451262..2452875 - 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -
K8B75_RS11850 (2452906) 2452906..2453256 - 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
K8B75_RS11855 (2453253) 2453253..2453678 - 426 WP_000422741.1 transposase -
K8B75_RS11860 (2454074) 2454074..2455396 - 1323 WP_001125411.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
K8B75_RS11865 (2455396) 2455396..2455662 - 267 WP_001296076.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
K8B75_RS11870 (2455970) 2455970..2456728 + 759 WP_001286537.1 trans-aconitate 2-methyltransferase -
K8B75_RS11875 (2456732) 2456732..2457646 - 915 WP_001296077.1 bestrophin family protein -
K8B75_RS11880 (2457843) 2457843..2459294 - 1452 WP_000854638.1 tagaturonate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(7-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49329.96 Da        Isoelectric Point: 7.7906

>T215310 WP_001125411.1 NZ_CP082831:c2455396-2454074 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAAEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHDRLSREYGIK

Download         Length: 1323 bp

>T215310 NZ_CP110634:1980238-1980414 [Bacillus subtilis]
GTGCTTGAGAAAGTGGGTATCATAGTTGCTTTCCTCATATCTTTAACGGTTCTTACAATCAACAGTCTAACAATAGTTGA
GAAGATAAGAAACCTAAAGAATGGGACAAGCAAAAAGAAAAAGCGTATACGCAAGCGGCTCCGACCAAAGAGACAACGCC
AACGTATACGCCGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9987.34 Da        Isoelectric Point: 4.9632

>AT215310 WP_001296076.1 NZ_CP082831:c2455662-2455396 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Download         Length: 267 bp

>AT215310 NZ_CP110634:c1980297-1980197 [Bacillus subtilis]
GATTGTAAGAACCGTTAAAGATATGAGGAAAGCAACTATGATACCCACTTTCTCAAGCACTATGTACACCTCCTTTCCTA
TGACTCTATTATAACATAATT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061YM43

Antitoxin

Source ID Structure
AlphaFold DB A0A061YNW4

References