Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1896014..1896845 Replicon chromosome
Accession NZ_CP082831
Organism Escherichia coli strain SCAID WND1-2021 (1/128)

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag K8B75_RS08930 Protein ID WP_000854815.1
Coordinates 1896014..1896388 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag K8B75_RS08935 Protein ID WP_001280918.1
Coordinates 1896477..1896845 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B75_RS08890 (1891410) 1891410..1892576 + 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
K8B75_RS08895 (1892695) 1892695..1893168 + 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
K8B75_RS08900 (1893366) 1893366..1894424 + 1059 WP_001200889.1 FUSC family protein -
K8B75_RS08905 (1894596) 1894596..1894925 + 330 WP_000450409.1 DUF496 family protein -
K8B75_RS27965 (1895026) 1895026..1895349 - 324 WP_225469267.1 EutP/PduV family microcompartment system protein -
K8B75_RS08915 (1895328) 1895328..1895408 + 81 WP_023441679.1 hypothetical protein -
K8B75_RS08920 (1895697) 1895697..1895777 - 81 Protein_1760 hypothetical protein -
K8B75_RS08925 (1895823) 1895823..1896017 - 195 WP_000988600.1 DUF5983 family protein -
K8B75_RS08930 (1896014) 1896014..1896388 - 375 WP_000854815.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
K8B75_RS08935 (1896477) 1896477..1896845 - 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
K8B75_RS08940 (1896861) 1896861..1897505 - 645 WP_000086752.1 hypothetical protein -
K8B75_RS08945 (1897524) 1897524..1897745 - 222 WP_000692345.1 DUF987 domain-containing protein -
K8B75_RS08950 (1897808) 1897808..1898284 - 477 WP_001186200.1 RadC family protein -
K8B75_RS08955 (1898300) 1898300..1898773 - 474 WP_001542276.1 antirestriction protein -
K8B75_RS08960 (1898867) 1898867..1899112 - 246 WP_001164966.1 hypothetical protein -
K8B75_RS08965 (1899112) 1899112..1899930 - 819 WP_001542275.1 DUF932 domain-containing protein -
K8B75_RS08970 (1900151) 1900151..1900561 - 411 WP_000846703.1 hypothetical protein -
K8B75_RS08975 (1900577) 1900577..1900927 - 351 Protein_1771 hypothetical protein -
K8B75_RS08980 (1901010) 1901010..1901756 - 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T215309 WP_000854815.1 NZ_CP082831:c1896388-1896014 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T215309 NZ_CP110634:c1401474-1400728 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATATACCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCCTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT215309 WP_001280918.1 NZ_CP082831:c1896845-1896477 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT215309 NZ_CP110634:c1400728-1400558 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAGAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References