Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 288820..289620 Replicon chromosome
Accession NZ_CP082831
Organism Escherichia coli strain SCAID WND1-2021 (1/128)

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag K8B75_RS01335 Protein ID WP_000342452.1
Coordinates 289093..289620 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag K8B75_RS01330 Protein ID WP_001277107.1
Coordinates 288820..289086 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B75_RS01310 (284479) 284479..285147 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
K8B75_RS01315 (285140) 285140..286198 + 1059 WP_001042013.1 permease-like cell division protein FtsX -
K8B75_RS01320 (286443) 286443..287297 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
K8B75_RS01325 (287568) 287568..288671 + 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
K8B75_RS01330 (288820) 288820..289086 + 267 WP_001277107.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
K8B75_RS01335 (289093) 289093..289620 + 528 WP_000342452.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
K8B75_RS01340 (289617) 289617..290000 - 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
K8B75_RS01345 (290423) 290423..291532 + 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
K8B75_RS01350 (291580) 291580..292506 + 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
K8B75_RS01355 (292503) 292503..293780 + 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
K8B75_RS01360 (293777) 293777..294544 + 768 WP_000082099.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T215303 WP_000342452.1 NZ_CP082831:289093-289620 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T215303 NZ_CP110622:2175187-2175492 [Prosthecochloris sp. SCSIO W1103]
ATGAATACTTTTATTCGATCAAACGAATTTGACCGATGGCTTTCCAACCTTTCAGACCAGAAAGCAAAAGCAAGAATCCT
GGCAAGACTTCGGAGTGCGATGCATGGCAATTTCGGTGATTGTGAGCCTGTCGGCGAAGGCGTATCGGAAATGCGCATCC
ATGTCGGAGCAGGTTATCGAGTATACTACACTCGAACAGGAACTACCGTTTACTTCCTGCTAGGCGGGGGCAGCAAGTCA
ACTCAGCGAAAAGATATCAAGAACCTAAAAAAACTAGCCAGGAAAATCAAGGAGACAGAACAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT215303 WP_001277107.1 NZ_CP082831:288820-289086 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT215303 NZ_CP110622:2175489-2175797 [Prosthecochloris sp. SCSIO W1103]
GTGAATAGCGAGTTTGCGACATTCGACATAGCCGATTATCTGGATAACGAAGAGGTCATTACCGAATATCTTTCCGCTGC
AACGGAAGATCCGAATCCTGATGTTTTTATTGCCGCTCTCGGTGACGTTGCTAAAGCCCGAGGCATGACACAGATCGCCA
AAGATTCGGGTTTAGGCAGGGAGAGTTTATATAAAGCGTTAAGCGCAGGATCACATCCACGATTTGAAACAATCAACGCT
GTCCTTCATGCTCTTGGTGTAAAGATTGCCATCATTCCAGCCGATAGGAGACAGAAAAAGACTTCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References