Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 120814..121339 Replicon plasmid unnamed1
Accession NZ_CP082828
Organism Escherichia coli strain SCAID WND2-2021 (3/145)

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag K8B73_RS25355 Protein ID WP_001159871.1
Coordinates 121034..121339 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag K8B73_RS25350 Protein ID WP_000813634.1
Coordinates 120814..121032 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B73_RS25345 (117851) 117851..118492 - 642 WP_223107155.1 hypothetical protein -
K8B73_RS25350 (120814) 120814..121032 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
K8B73_RS25355 (121034) 121034..121339 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
K8B73_RS25360 (121340) 121340..122149 + 810 WP_000016979.1 site-specific integrase -
K8B73_RS25365 (122287) 122287..122562 - 276 WP_000239529.1 hypothetical protein -
K8B73_RS25370 (122556) 122556..123200 - 645 WP_000633911.1 AAA family ATPase -
K8B73_RS25375 (123429) 123429..124399 + 971 Protein_150 plasmid segregation protein ParM -
K8B73_RS25380 (124404) 124404..124796 + 393 WP_000340835.1 plasmid partitioning/stability family protein -
K8B73_RS25385 (124801) 124801..125052 - 252 Protein_152 DUF4113 domain-containing protein -
K8B73_RS25390 (125114) 125114..125911 - 798 WP_001703540.1 IS21-like element IS21 family helper ATPase IstB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / sul1 / blaTEM-1B / catA1 / mph(A) / tet(B) senB 1..139267 139267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T215296 WP_001159871.1 NZ_CP082828:121034-121339 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T215296 NZ_CP110620:c884347-884063 [Pasteurella multocida]
GTGGGTCGGTTAAGGGAGGTAAAAAATTTATCCGCCAAAGCCGCTGTTCTTGCTCGTATTAACAGAGTAATGAATGGTAA
TTTGGGCGATCATAAATCGATTGGAAATGGATTGTATGAGATGAGAATTATAAAAGGTTCGGGTTATAGAGTTTACTATG
GACAATATAGAGAAGTAACTTATTTATTAATTTGTGGTGGCGATAAATCAACACAGAAATCAGACATTGTTAAAGCGAGA
GAGTTGTGGAAAGAAATAAAGCAACAAGAGGAAGTAAAAGTATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT215296 WP_000813634.1 NZ_CP082828:120814-121032 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT215296 NZ_CP110620:c884066-883773 [Pasteurella multocida]
ATGAGTAAGAAAAAAATAAAATTAAAACTGTTTGATATTGCTGAACATCTAGACAGTGAAGAAATGATTGCGGCATATTT
AAGTGAAGTTTTAGAAACATGTGATACTAATGAATTTATTTCCGCTTTAGCTGATGTTGTAAGAGCAAAAGGAATGACGG
AGTTAGCAGCAAAAACAGGGTTAAGAAGAGAAAGCCTCTATAAAACATTATCATACGGTAGCAAGCCACGTTTTGATACG
ATAATGAAAATTACGCATTCATTAGGTATTAAACTTGTCCCAACGCACATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References