215296

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 120814..121339 Replicon plasmid unnamed1
Accession NZ_CP082828
Organism Escherichia coli strain SCAID WND2-2021 (3/145)

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag K8B73_RS25355 Protein ID WP_001159871.1
Coordinates 121034..121339 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag K8B73_RS25350 Protein ID WP_000813634.1
Coordinates 120814..121032 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B73_RS25345 (117851) 117851..118492 - 642 WP_223107155.1 hypothetical protein -
K8B73_RS25350 (120814) 120814..121032 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
K8B73_RS25355 (121034) 121034..121339 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
K8B73_RS25360 (121340) 121340..122149 + 810 WP_000016979.1 site-specific integrase -
K8B73_RS25365 (122287) 122287..122562 - 276 WP_000239529.1 hypothetical protein -
K8B73_RS25370 (122556) 122556..123200 - 645 WP_000633911.1 AAA family ATPase -
K8B73_RS25375 (123429) 123429..124399 + 971 Protein_150 plasmid segregation protein ParM -
K8B73_RS25380 (124404) 124404..124796 + 393 WP_000340835.1 plasmid partitioning/stability family protein -
K8B73_RS25385 (124801) 124801..125052 - 252 Protein_152 DUF4113 domain-containing protein -
K8B73_RS25390 (125114) 125114..125911 - 798 WP_001703540.1 IS21-like element IS21 family helper ATPase IstB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / sul1 / blaTEM-1B / catA1 / mph(A) / tet(B) senB 1..139267 139267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T215296 WP_001159871.1 NZ_CP082828:121034-121339 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T215296 NZ_CP110620:c884347-884063 [Pasteurella multocida]
GTGGGTCGGTTAAGGGAGGTAAAAAATTTATCCGCCAAAGCCGCTGTTCTTGCTCGTATTAACAGAGTAATGAATGGTAA
TTTGGGCGATCATAAATCGATTGGAAATGGATTGTATGAGATGAGAATTATAAAAGGTTCGGGTTATAGAGTTTACTATG
GACAATATAGAGAAGTAACTTATTTATTAATTTGTGGTGGCGATAAATCAACACAGAAATCAGACATTGTTAAAGCGAGA
GAGTTGTGGAAAGAAATAAAGCAACAAGAGGAAGTAAAAGTATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT215296 WP_000813634.1 NZ_CP082828:120814-121032 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT215296 NZ_CP110620:c884066-883773 [Pasteurella multocida]
ATGAGTAAGAAAAAAATAAAATTAAAACTGTTTGATATTGCTGAACATCTAGACAGTGAAGAAATGATTGCGGCATATTT
AAGTGAAGTTTTAGAAACATGTGATACTAATGAATTTATTTCCGCTTTAGCTGATGTTGTAAGAGCAAAAGGAATGACGG
AGTTAGCAGCAAAAACAGGGTTAAGAAGAGAAAGCCTCTATAAAACATTATCATACGGTAGCAAGCCACGTTTTGATACG
ATAATGAAAATTACGCATTCATTAGGTATTAAACTTGTCCCAACGCACATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References