Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4659368..4659865 Replicon chromosome
Accession NZ_CP082827
Organism Escherichia coli strain SCAID WND2-2021 (3/145)

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag K8B73_RS22475 Protein ID WP_001173343.1
Coordinates 4659368..4659541 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag K8B73_RS22480 Protein ID WP_000398619.1
Coordinates 4659569..4659865 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B73_RS22465 (4656039) 4656039..4657496 + 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -
K8B73_RS22470 (4657733) 4657733..4659250 + 1518 WP_000003806.1 lysine--tRNA ligase -
K8B73_RS22475 (4659368) 4659368..4659541 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
K8B73_RS22480 (4659569) 4659569..4659865 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
K8B73_RS22485 (4660094) 4660094..4660366 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
K8B73_RS22490 (4660378) 4660378..4660608 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
K8B73_RS22495 (4660789) 4660789..4662420 + 1632 WP_001216477.1 sensor histidine kinase -
K8B73_RS22500 (4662417) 4662417..4663136 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4650515..4659865 9350


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T215295 WP_001173343.1 NZ_CP082827:c4659541-4659368 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T215295 NZ_CP110620:c884066-883773 [Pasteurella multocida]
ATGAGTAAGAAAAAAATAAAATTAAAACTGTTTGATATTGCTGAACATCTAGACAGTGAAGAAATGATTGCGGCATATTT
AAGTGAAGTTTTAGAAACATGTGATACTAATGAATTTATTTCCGCTTTAGCTGATGTTGTAAGAGCAAAAGGAATGACGG
AGTTAGCAGCAAAAACAGGGTTAAGAAGAGAAAGCCTCTATAAAACATTATCATACGGTAGCAAGCCACGTTTTGATACG
ATAATGAAAATTACGCATTCATTAGGTATTAAACTTGTCCCAACGCACATCTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT215295 WP_000398619.1 NZ_CP082827:c4659865-4659569 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT215295 NZ_CP110620:c884347-884063 [Pasteurella multocida]
GTGGGTCGGTTAAGGGAGGTAAAAAATTTATCCGCCAAAGCCGCTGTTCTTGCTCGTATTAACAGAGTAATGAATGGTAA
TTTGGGCGATCATAAATCGATTGGAAATGGATTGTATGAGATGAGAATTATAAAAGGTTCGGGTTATAGAGTTTACTATG
GACAATATAGAGAAGTAACTTATTTATTAATTTGTGGTGGCGATAAATCAACACAGAAATCAGACATTGTTAAAGCGAGA
GAGTTGTGGAAAGAAATAAAGCAACAAGAGGAAGTAAAAGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References