215286

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3765986..3766604 Replicon chromosome
Accession NZ_CP082827
Organism Escherichia coli strain SCAID WND2-2021 (3/145)

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag K8B73_RS18230 Protein ID WP_001291435.1
Coordinates 3766386..3766604 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag K8B73_RS18225 Protein ID WP_000344800.1
Coordinates 3765986..3766360 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B73_RS18215 (3761075) 3761075..3762268 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K8B73_RS18220 (3762291) 3762291..3765440 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
K8B73_RS18225 (3765986) 3765986..3766360 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
K8B73_RS18230 (3766386) 3766386..3766604 + 219 WP_001291435.1 HHA domain-containing protein Toxin
K8B73_RS18235 (3766776) 3766776..3767327 + 552 WP_000102559.1 maltose O-acetyltransferase -
K8B73_RS18240 (3767443) 3767443..3767913 + 471 WP_000136192.1 YlaC family protein -
K8B73_RS18245 (3768077) 3768077..3769627 + 1551 WP_001389864.1 cyclic-guanylate-specific phosphodiesterase PdeB -
K8B73_RS18250 (3769669) 3769669..3770022 - 354 WP_000878140.1 DUF1428 domain-containing protein -
K8B73_RS18260 (3770401) 3770401..3770712 + 312 WP_000409911.1 MGMT family protein -
K8B73_RS18265 (3770743) 3770743..3771315 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T215286 WP_001291435.1 NZ_CP082827:3766386-3766604 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T215286 NZ_CP110619:3181461-3181724 [Mycobacterium tuberculosis]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGCATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT215286 WP_000344800.1 NZ_CP082827:3765986-3766360 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT215286 NZ_CP110619:3181176-3181457 [Mycobacterium tuberculosis]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References