Detailed information of TA system TA215285

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3275779..3276484
• Replicon: chromosome
• Accession: NZ_CP082827
• Organism: Escherichia coli strain SCAID WND2-2021 (3/145)

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: K8B73_RS15800
• Protein ID: WP_000539521.1
• Coordinates: 3275779..3276165 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: K8B73_RS15805
• Protein ID: WP_001280945.1
• Coordinates: 3276155..3276484 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B73_RS15780 (3271783)	3271783..3272409	+	627	WP_001295292.1	glutathione S-transferase GstB	-
K8B73_RS15785 (3272406)	3272406..3273521	-	1116	WP_000555038.1	aldose sugar dehydrogenase YliI	-
K8B73_RS15790 (3273632)	3273632..3274015	-	384	WP_000497137.1	biofilm formation regulator BssR	-
K8B73_RS15795 (3274228)	3274228..3275553	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
K8B73_RS15800 (3275779)	3275779..3276165	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K8B73_RS15805 (3276155)	3276155..3276484	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
K8B73_RS15810 (3276554)	3276554..3277882	-	1329	WP_000086879.1	GGDEF domain-containing protein	-
K8B73_RS15815 (3277890)	3277890..3280238	-	2349	WP_001322378.1	EAL domain-containing protein	-
K8B73_RS15820 (3280416)	3280416..3281327	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T215285 WP_000539521.1 NZ_CP082827:3275779-3276165 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T215285 NZ_CP110619:c3140651-3140259 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT215285 WP_001280945.1 NZ_CP082827:3276155-3276484 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT215285 NZ_CP110619:c3140863-3140648 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGCGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6