Detailed information of TA system TA215283

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2629830..2630468
• Replicon: chromosome
• Accession: NZ_CP082827
• Organism: Escherichia coli strain SCAID WND2-2021 (3/145)

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: K8B73_RS12470
• Protein ID: WP_000813797.1
• Coordinates: 2630292..2630468 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K8B73_RS12465
• Protein ID: WP_076611057.1
• Coordinates: 2629830..2630246 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B73_RS12445 (2624982)	2624982..2625923	-	942	WP_001251335.1	ABC transporter permease	-
K8B73_RS12450 (2625924)	2625924..2626937	-	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
K8B73_RS12455 (2626955)	2626955..2628100	-	1146	WP_000047447.1	ABC transporter substrate-binding protein	-
K8B73_RS12460 (2628345)	2628345..2629751	-	1407	WP_000760613.1	PLP-dependent aminotransferase family protein	-
K8B73_RS12465 (2629830)	2629830..2630246	-	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
K8B73_RS12470 (2630292)	2630292..2630468	-	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
K8B73_RS12475 (2630690)	2630690..2630920	+	231	WP_000494244.1	YncJ family protein	-
K8B73_RS12480 (2631012)	2631012..2632973	-	1962	WP_001309488.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
K8B73_RS12485 (2633046)	2633046..2633582	-	537	WP_000429161.1	DNA-binding transcriptional regulator SutR	-
K8B73_RS12490 (2633635)	2633635..2634846	+	1212	WP_001323162.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T215283 WP_000813797.1 NZ_CP082827:c2630468-2630292 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T215283 NZ_CP110619:c3114162-3113806 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT215283 WP_076611057.1 NZ_CP082827:c2630246-2629830 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT215283 NZ_CP110619:c3114376-3114146 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• AlphaFold DB: A0A829J715