Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | sanaTA/AbiEii-DUF6088 |
| Location | 845897..847178 | Replicon | chromosome |
| Accession | NZ_CP082827 | ||
| Organism | Escherichia coli strain SCAID WND2-2021 (3/145) | ||
Toxin (Protein)
| Gene name | sanaT | Uniprot ID | A0A1X3JHM4 |
| Locus tag | K8B73_RS04045 | Protein ID | WP_001114681.1 |
| Coordinates | 845897..846790 (-) | Length | 298 a.a. |
Antitoxin (Protein)
| Gene name | sanaA | Uniprot ID | V0YCI1 |
| Locus tag | K8B73_RS04050 | Protein ID | WP_000208383.1 |
| Coordinates | 846783..847178 (-) | Length | 132 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| K8B73_RS04020 (841081) | 841081..841820 | + | 740 | Protein_793 | IS3 family transposase | - |
| K8B73_RS04025 (842493) | 842493..844001 | + | 1509 | WP_001189111.1 | group II intron reverse transcriptase/maturase | - |
| K8B73_RS04030 (844085) | 844085..844564 | + | 480 | Protein_795 | IS3 family transposase | - |
| K8B73_RS04035 (844605) | 844605..844907 | - | 303 | Protein_796 | IS21 family transposase | - |
| K8B73_RS04040 (845256) | 845256..845426 | - | 171 | Protein_797 | IS110 family transposase | - |
| K8B73_RS04045 (845897) | 845897..846790 | - | 894 | WP_001114681.1 | nucleotidyl transferase AbiEii/AbiGii toxin family protein | Toxin |
| K8B73_RS04050 (846783) | 846783..847178 | - | 396 | WP_000208383.1 | DUF6088 family protein | Antitoxin |
| K8B73_RS04055 (847247) | 847247..848092 | - | 846 | WP_001529401.1 | DUF4942 domain-containing protein | - |
| K8B73_RS04060 (848177) | 848177..848374 | - | 198 | WP_000839293.1 | DUF957 domain-containing protein | - |
| K8B73_RS04065 (848391) | 848391..848879 | - | 489 | WP_000761699.1 | DUF5983 family protein | - |
| K8B73_RS04070 (848876) | 848876..849253 | - | 378 | WP_000854689.1 | TA system toxin CbtA family protein | - |
| K8B73_RS04075 (849330) | 849330..849710 | - | 381 | WP_223107141.1 | type IV toxin-antitoxin system YeeU family antitoxin | - |
| K8B73_RS04080 (849760) | 849760..850404 | - | 645 | WP_021566525.1 | hypothetical protein | - |
| K8B73_RS04085 (850423) | 850423..850644 | - | 222 | WP_000692300.1 | DUF987 domain-containing protein | - |
| K8B73_RS04090 (850707) | 850707..851183 | - | 477 | WP_001186774.1 | RadC family protein | - |
| K8B73_RS04095 (851198) | 851198..851683 | - | 486 | WP_000849565.1 | antirestriction protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 298 a.a. Molecular weight: 34298.40 Da Isoelectric Point: 5.9850
>T215270 WP_001114681.1 NZ_CP082827:c846790-845897 [Escherichia coli]
MPELTSLFPDVADALDIESVAIVEKDYFVVDLLRLLKEIKPETHTLVFAGSTALSKAGISLNRMSEDIDIKLVPTENFMQ
NGRDKRRKIRKEIVQIITDVIHNSDIFSLDNENARITRDEYRYNEISVRYPQTFAQVPCLRPFIKLELMESTLLEHPESR
DIYSLVTELTGKGTPITAFPCATILSTQAEKLISMMRRTAAHLRNPEQQDDEFLVRHIYDNYCIVREKGVNVPVLKNFVQ
ICIQQDIERYGNQYPQFCSSPVDELKKGLDALRDYPVHKLRFQRFVNPMVFGNTQIN
MPELTSLFPDVADALDIESVAIVEKDYFVVDLLRLLKEIKPETHTLVFAGSTALSKAGISLNRMSEDIDIKLVPTENFMQ
NGRDKRRKIRKEIVQIITDVIHNSDIFSLDNENARITRDEYRYNEISVRYPQTFAQVPCLRPFIKLELMESTLLEHPESR
DIYSLVTELTGKGTPITAFPCATILSTQAEKLISMMRRTAAHLRNPEQQDDEFLVRHIYDNYCIVREKGVNVPVLKNFVQ
ICIQQDIERYGNQYPQFCSSPVDELKKGLDALRDYPVHKLRFQRFVNPMVFGNTQIN
Download Length: 894 bp
>T215270 NZ_CP110619:2259846-2260244 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA
Antitoxin
Download Length: 132 a.a. Molecular weight: 14608.03 Da Isoelectric Point: 11.3150
>AT215270 WP_000208383.1 NZ_CP082827:c847178-846783 [Escherichia coli]
MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEGKLMKVGYGVYTKARRNIITGKIMPAAPGGSAAVITE
ALERLNIRYRLAGATECYNKGQSTQIPVSLGIKTPSRFKRVLIVGKSKINA
MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEGKLMKVGYGVYTKARRNIITGKIMPAAPGGSAAVITE
ALERLNIRYRLAGATECYNKGQSTQIPVSLGIKTPSRFKRVLIVGKSKINA
Download Length: 396 bp
>AT215270 NZ_CP110619:2259618-2259845 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1X3JHM4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0YCI1 |