Detailed information of TA system TA215266

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 150299..150911
• Replicon: chromosome
• Accession: NZ_CP082827
• Organism: Escherichia coli strain SCAID WND2-2021 (3/145)

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: K8B73_RS00645
• Protein ID: WP_000833473.1
• Coordinates: 150299..150484 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: K8B73_RS00650
• Protein ID: WP_000499744.1
• Coordinates: 150501..150911 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B73_RS00635 (147131)	147131..148282	+	1152	WP_000741501.1	L-threonine dehydrogenase	-
K8B73_RS00640 (148483)	148483..150021	+	1539	WP_001309845.1	aldehyde dehydrogenase AldB	-
K8B73_RS00645 (150299)	150299..150484	+	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
K8B73_RS00650 (150501)	150501..150911	+	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
K8B73_RS00655 (150983)	150983..152947	-	1965	WP_001026861.1	glycoside hydrolase family 127 protein	-
K8B73_RS00660 (152958)	152958..154358	-	1401	WP_000204802.1	MFS transporter	-
K8B73_RS00665 (154584)	154584..155399	+	816	WP_000891825.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T215266 WP_000833473.1 NZ_CP082827:150299-150484 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T215266 NZ_CP110619:c2205549-2205253 [Mycobacterium tuberculosis]
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGAGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT215266 WP_000499744.1 NZ_CP082827:150501-150911 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT215266 NZ_CP110619:c2205797-2205546 [Mycobacterium tuberculosis]
GTGGGTAAGAACACGTCCTTCGTCCTCGACGAGCACTACAGCGCCTTCATCGACGGCGAGATCGCCGCGGGCCGCTACCG
GTCGGCCAGTGAAGTCATCCGCTCCGCGTTGCGACTGCTCGAGGACCGTGAAACCCAGCTGCGCGCGCTTCGTGAGGCTC
TCGAGGCCGGCGAACGCAGCGGCAGCTCGACACCGTTCGACTTCGACGGATTCCTCGGTCGTAAGCGGGCTGACGCCTCG
CGTGGCCGGTGA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9