Detailed information of TA system TA215261

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 12218..12743
• Replicon: plasmid unnamed1
• Accession: NZ_CP082825
• Organism: Escherichia coli strain SCAID URN1-2021 (19/278)

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: V0SSI5
• Locus tag: K8B79_RS25275
• Protein ID: WP_001159868.1
• Coordinates: 12438..12743 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1PPD8
• Locus tag: K8B79_RS25270
• Protein ID: WP_000813634.1
• Coordinates: 12218..12436 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B79_RS25240 (K8B79_25240)	7611..8027	-	417	WP_001034046.1	type II toxin-antitoxin system VapC family toxin	-
K8B79_RS25245 (K8B79_25245)	8024..8254	-	231	WP_001261278.1	type II toxin-antitoxin system VapB family antitoxin	-
K8B79_RS25250 (K8B79_25250)	8519..9019	+	501	WP_001773886.1	HEPN family nuclease	-
K8B79_RS25255 (K8B79_25255)	9032..9805	+	774	WP_000905949.1	hypothetical protein	-
K8B79_RS25260 (K8B79_25260)	9972..11105	+	1134	WP_001542067.1	DUF3800 domain-containing protein	-
K8B79_RS25265 (K8B79_25265)	11139..11651	-	513	WP_000151784.1	hypothetical protein	-
K8B79_RS25270 (K8B79_25270)	12218..12436	+	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
K8B79_RS25275 (K8B79_25275)	12438..12743	+	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	Toxin
K8B79_RS25280 (K8B79_25280)	12744..13550	+	807	WP_000016982.1	site-specific integrase	-
K8B79_RS25285 (K8B79_25285)	14324..15079	+	756	WP_000852146.1	replication initiation protein RepE	-
K8B79_RS25290 (K8B79_25290)	15667..16833	+	1167	WP_000772446.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	dfrA17 / tet(B) / sul2 / aph(3'')-Ib / aph(6)-Id / mph(A) / blaTEM-1B	senB	1..108070	108070

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11706.51 Da
• Isoelectric Point: 6.4674

>T215261 WP_001159868.1 NZ_CP082825:12438-12743 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T215261 NZ_CP110619:1765192-1765596 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.34 Da
• Isoelectric Point: 4.7405

>AT215261 WP_000813634.1 NZ_CP082825:12218-12436 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT215261 NZ_CP110619:1764983-1765186 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Toxin

• PDB: 4VUB
• PDB: 2VUB
• PDB: 7EPI
• PDB: 7EPG
• PDB: 3VUB
• PDB: 1X75
• PDB: 1VUB
• PDB: 7EPJ
• PDB: 3HPW
• PDB: 3G7Z

Antitoxin

• PDB: 3TCJ
• PDB: 2H3A
• PDB: 2ADN
• PDB: 2ADL
• PDB: 3HPW
• PDB: 2H3C
• PDB: 3G7Z
• AlphaFold DB: A0A829CQY2