215260

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 7611..8254 Replicon plasmid unnamed1
Accession NZ_CP082825
Organism Escherichia coli strain SCAID URN1-2021 (19/278)

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag K8B79_RS25240 Protein ID WP_001034046.1
Coordinates 7611..8027 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag K8B79_RS25245 Protein ID WP_001261278.1
Coordinates 8024..8254 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B79_RS25225 (K8B79_25225) 2748..3164 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
K8B79_RS25230 (K8B79_25230) 3161..3391 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
K8B79_RS25235 (K8B79_25235) 3772..7566 + 3795 WP_001144732.1 hypothetical protein -
K8B79_RS25240 (K8B79_25240) 7611..8027 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
K8B79_RS25245 (K8B79_25245) 8024..8254 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K8B79_RS25250 (K8B79_25250) 8519..9019 + 501 WP_001773886.1 HEPN family nuclease -
K8B79_RS25255 (K8B79_25255) 9032..9805 + 774 WP_000905949.1 hypothetical protein -
K8B79_RS25260 (K8B79_25260) 9972..11105 + 1134 WP_001542067.1 DUF3800 domain-containing protein -
K8B79_RS25265 (K8B79_25265) 11139..11651 - 513 WP_000151784.1 hypothetical protein -
K8B79_RS25270 (K8B79_25270) 12218..12436 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
K8B79_RS25275 (K8B79_25275) 12438..12743 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA17 / tet(B) / sul2 / aph(3'')-Ib / aph(6)-Id / mph(A) / blaTEM-1B senB 1..108070 108070


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T215260 WP_001034046.1 NZ_CP082825:c8027-7611 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T215260 NZ_CP110619:1686783-1687100 [Mycobacterium tuberculosis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT215260 WP_001261278.1 NZ_CP082825:c8254-8024 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT215260 NZ_CP110619:1686484-1686786 [Mycobacterium tuberculosis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References