Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 2748..3391 Replicon plasmid unnamed1
Accession NZ_CP082825
Organism Escherichia coli strain SCAID URN1-2021 (19/278)

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag K8B79_RS25225 Protein ID WP_001034044.1
Coordinates 2748..3164 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag K8B79_RS25230 Protein ID WP_001261286.1
Coordinates 3161..3391 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K8B79_RS25215 (K8B79_25215) 101..1123 - 1023 WP_000361402.1 helicase UvrD -
K8B79_RS25220 (K8B79_25220) 1108..2673 - 1566 WP_001128474.1 AAA family ATPase -
K8B79_RS25225 (K8B79_25225) 2748..3164 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
K8B79_RS25230 (K8B79_25230) 3161..3391 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K8B79_RS25235 (K8B79_25235) 3772..7566 + 3795 WP_001144732.1 hypothetical protein -
K8B79_RS25240 (K8B79_25240) 7611..8027 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
K8B79_RS25245 (K8B79_25245) 8024..8254 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA17 / tet(B) / sul2 / aph(3'')-Ib / aph(6)-Id / mph(A) / blaTEM-1B senB 1..108070 108070


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T215259 WP_001034044.1 NZ_CP082825:c3164-2748 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T215259 NZ_CP110619:c1574519-1574118 [Mycobacterium tuberculosis]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGACCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT215259 WP_001261286.1 NZ_CP082825:c3391-3161 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT215259 NZ_CP110619:c1574773-1574516 [Mycobacterium tuberculosis]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References