Detailed information of TA system TA215257

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4601074..4601906
• Replicon: chromosome
• Accession: NZ_CP082824
• Organism: Escherichia coli strain SCAID URN1-2021 (19/278)

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: K8B79_RS22570
• Protein ID: WP_000854753.1
• Coordinates: 4601532..4601906 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NQ68
• Locus tag: K8B79_RS22565
• Protein ID: WP_001540478.1
• Coordinates: 4601074..4601442 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K8B79_RS22530 (4596907)	4596907..4597587	+	681	WP_001282919.1	WYL domain-containing protein	-
K8B79_RS22535 (4597735)	4597735..4598412	+	678	WP_001097301.1	hypothetical protein	-
K8B79_RS22540 (4598418)	4598418..4598651	+	234	WP_001278283.1	DUF905 family protein	-
K8B79_RS22545 (4598741)	4598741..4599559	+	819	WP_001773857.1	DUF932 domain-containing protein	-
K8B79_RS22550 (4599650)	4599650..4600135	+	486	WP_000214398.1	antirestriction protein	-
K8B79_RS22555 (4600151)	4600151..4600627	+	477	WP_001186786.1	RadC family protein	-
K8B79_RS22560 (4600690)	4600690..4600911	+	222	WP_000692311.1	DUF987 domain-containing protein	-
K8B79_RS22565 (4601074)	4601074..4601442	+	369	WP_001540478.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K8B79_RS22570 (4601532)	4601532..4601906	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
K8B79_RS22575 (4601903)	4601903..4602391	+	489	WP_000777545.1	DUF5983 family protein	-
K8B79_RS22580 (4602403)	4602403..4602600	+	198	WP_000839281.1	DUF957 domain-containing protein	-
K8B79_RS22585 (4602697)	4602697..4603266	+	570	WP_001560692.1	DUF4942 domain-containing protein	-
K8B79_RS22590 (4604015)	4604015..4605553	+	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4557142..4613366	56224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T215257 WP_000854753.1 NZ_CP082824:4601532-4601906 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T215257 NZ_CP110619:c1388984-1388691 [Mycobacterium tuberculosis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13652.53 Da
• Isoelectric Point: 7.8398

>AT215257 WP_001540478.1 NZ_CP082824:4601074-4601442 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT215257 NZ_CP110619:c1389250-1388981 [Mycobacterium tuberculosis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: S1NQ68